Reference : Phase I/II trial of immunogenicity of a human papillomavirus (HPV) type 16 E7 protein...
Scientific journals : Article
Human health sciences : Oncology
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/5212
Phase I/II trial of immunogenicity of a human papillomavirus (HPV) type 16 E7 protein-based vaccine in women with oncogenic HPV-positive cervical intraepithelial neoplasia
English
Hallez, Sophie [> > > >]
Simon, Philippe [Department of Obstetrics and Gynaecology, Hopital Erasme,]
Maudoux, Frédéric [> > > >]
Doyen, Jean [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
Noël, Jean-Christophe [> > > >]
Beliard, Aude mailto [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
Capelle, Xavier mailto [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique >]
Buxant, Frédéric [> > > >]
Fayt, Isabelle [> > > >]
Lagrost, Anne-Cécile [> > > >]
Hubert, Pascale mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Gerday, Colette [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
Burny, Arsène [> > > >]
Boniver, Jacques mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique >]
Delvenne, Philippe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Jacobs, Nathalie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Jul-2004
Cancer Immunology, Immunotherapy
Springer Verlag
53
7
642-650
Yes (verified by ORBi)
International
0340-7004
New York
[en] CD4 ; CD8 ; human papillomavirus ; IFN-gamma ; immunotherapy
[en] Purpose: Infection with oncogenic human papillomavirus (HPV) and HPV-16 in particular is a leading cause of anogenital neoplasia. High-grade intraepithelial lesions require treatment because of their potential to progress to invasive cancer. Numerous preclinical studies have demonstrated the therapeutic potential of E7-directed vaccination strategies in mice tumour models. In the present study, we tested the immunogenicity of a fusion protein (PD-E7) comprising a mutated HPV-16 E7 linked to the first 108 amino acids of Haemophilus influenzae protein D, formulated in the GlaxoSmithKline Biologicals adjuvant AS02B, in patients bearing oncogenic HPV-positive cervical intraepithelial neoplasia (CIN). Methods: Seven patients, five with a CIN3 and two with a CIN1, received three intramuscular injections of adjuvanted PD-E7 at 2-week intervals. Three additional CIN1 patients received a placebo. CIN3 patients underwent conization 8 weeks postvaccination. Cytokine flow cytometry and ELISA were used to monitor antigen-specific cellular and antibody responses from blood taken before and after vaccine or placebo injection. Results: Some patients had preexisting systemic IFN-gamma CD4(+) (1/10) and CD8(+) (5/10) responses to PD-E7. Vaccination, not placebo injection, elicited systemic specific immune responses in the majority of the patients. Five vaccinated patients (71%) showed significantly increased IFN-gamma CD8(+) cell responses upon PD-E7 stimulation. Two responding patients generated long-term T-cell immunity toward the vaccine antigen and E7 as well as a weak H. influenzae protein D (PD)-directed CD4(+) response. All the vaccinated patients, but not the placebo, made significant E7- and PD-specific IgG. Conclusions: The encouraging results obtained from this study performed on a limited number of subjects justify further analysis of the efficacy of the PD-E7/AS02B vaccine in CIN patients.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE ; Yvonne Boel Foundation
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/5212
http://www.springerlink.com/content/e9ttuu985wh9xh9b/
The original publication is available at www.springerlink.com

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
Hallez S CII 2004.pdf.pdfAuthor postprint602.41 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.