Article (Scientific journals)
Myeloperoxidase Assay in Plasma and Peritoneal Fluid of Horses with Gastrointestinal Disease
Grulke, Sigrid; Franck, Thierry; Gangl, Monika et al.
2008In Canadian Journal of Veterinary Research, 72 (1), p. 37-42
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Keywords :
horse; Colic; myeloperoxidase; peritoneal fluid
Abstract :
[en] Gastrointestinal disorders, especially strangulating intestinal obstructions, are still a major cause of illness and death in the horse. Circulating lipopolysaccharides may activate both neutrophils and monocytes. The activated neutrophils release myeloperoxidase (MPO), a specific enzyme with strong oxidative activity. The aim of this study was to evaluate MPO concentrations in the plasma and peritoneal fluid (PF) of horses with colic and to check the hypothesis that these concentrations would be higher in a case of strangulating obstruction than in cases of nonstrangulating disease. By using a specific enzyme-linked immunosorbent assay for equine MPO, we determined the MPO concentrations in horses admitted to a clinic for colic. Horses with nonstrangulating or strangulating obstruction of the large intestine (NSLI or SLI), strangulating obstruction of the small intestine (SSI), or inflammatory bowel disease (IBD) were compared with healthy horses. The horses with SLI, SSI, or IBD had significantly higher MPO levels in plasma and PF than did those in the other 2 groups. The mean plasma level was significantly higher in the horses with NSLI than in the healthy horses. High MPO values in PF indicated necrotic bowel. These results show that neutrophil activation occurs during nonstrangulating and strangulating intestinal obstruction in horses and that the plasma and PF MPO concentrations may be a marker of the severity of the disease.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Grulke, Sigrid  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Franck, Thierry  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Gangl, Monika
Péters, Fabrice
Salciccia, Alexandra  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Deby, Ginette ;  Centre Hospitalier Universitaire de Liège - CHU > Anesthésie et réanimation
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Myeloperoxidase Assay in Plasma and Peritoneal Fluid of Horses with Gastrointestinal Disease
Publication date :
January 2008
Journal title :
Canadian Journal of Veterinary Research
ISSN :
0830-9000
Publisher :
Canadian Veterinary Medical Association, Canada
Volume :
72
Issue :
1
Pages :
37-42
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 January 2009

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