Reference : Discrimination between Bifidobacterium species from human and animal origin by PCR-re...
Scientific journals : Article
Life sciences : Food science
Life sciences : Microbiology
http://hdl.handle.net/2268/514
Discrimination between Bifidobacterium species from human and animal origin by PCR-restriction fragment length polymorphism
English
Delcenserie, Véronique mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaire >]
Bechoux, Nathalie mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires >]
Leonard, Tamara [Université de Liège - ULg > Sciences des Denrées alimentaires > Microbiologie des Denrées alimentaires > >]
China, Bernard [Université de Liège - ULg > Sciences des Denrées alimentaires > Microbiologie des Denrées alimentaires > >]
Daube, Georges mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires >]
2004
Journal of Food Protection
Int Assoc Food Protection
67
6
1284-1288
Yes (verified by ORBi)
International
0362-028X
Des Moines
[en] Bifidobacterium ; PCR-RFLP ; Typing
[en] Bifidobacteria are normal intestinal flora in humans and animals. The genus Bifidobacterium includes 31 species of significant host specificity. Taking into account their properties, we proposed to use bifidobacteria as fecal contamination indicators. PCR-restriction fragment length polymorphism on the 16S rDNA gene was used to distinguish the different Bifidobacterium species. Sixty-four strains belonging to 13 different species were differentiated from animal or human origin using one or two restriction enzymes. Moreover, the primers used were specifics of the Bifidobacterium genus. Therefore, this method made it possible to determine both the presence of bifidobacteria in a sample and its origin of contamination.
Researchers ; Students
http://hdl.handle.net/2268/514
also: http://hdl.handle.net/2268/111334

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