ORBi: Delcenserie Véronique - Discrimination between Bifidobacterium species from human and animal origin by PCR-restriction fragment length polymorphism

Reference : Discrimination between Bifidobacterium species from human and animal origin by PCR-restr...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Food science Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/2268/514

Title :	Discrimination between Bifidobacterium species from human and animal origin by PCR-restriction fragment length polymorphism
Language :	English
Author, co-author :	Delcenserie, Véronique [Université de Liège - ULg > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaire >]
	Bechoux, Nathalie [Université de Liège - ULg > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires >]
	Leonard, Tamara [Université de Liège - ULg > Sciences des Denrées alimentaires > Microbiologie des Denrées alimentaires > >]
	China, Bernard [Université de Liège - ULg > Sciences des Denrées alimentaires > Microbiologie des Denrées alimentaires > >]
	Daube, Georges [Université de Liège - ULg > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires >]
Publication date :	2004
Journal title :	Journal of Food Protection
Publisher :	Int Assoc Food Protection
Volume :	67
Issue/season :	6
Pages :	1284-1288
Audience :	International
ISSN :	0362-028X
City :	Des Moines
Keywords :	[en] Bifidobacterium ; PCR-RFLP ; Typing
Abstract :	[en] Bifidobacteria are normal intestinal flora in humans and animals. The genus Bifidobacterium includes 31 species of significant host specificity. Taking into account their properties, we proposed to use bifidobacteria as fecal contamination indicators. PCR-restriction fragment length polymorphism on the 16S rDNA gene was used to distinguish the different Bifidobacterium species. Sixty-four strains belonging to 13 different species were differentiated from animal or human origin using one or two restriction enzymes. Moreover, the primers used were specifics of the Bifidobacterium genus. Therefore, this method made it possible to determine both the presence of bifidobacteria in a sample and its origin of contamination.
Target :	Researchers ; Students
Permalink :	http://hdl.handle.net/2268/514
	also: http://hdl.handle.net/2268/111334

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	File	Commentary	Version	Size	Access
Open access	JFP-03-402_BIFIDO_auteur.pdf		Author preprint	2.97 MB	View/Open

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