Article (Scientific journals)
Identification of Sox17 as a transcription factor that regulates oligodendrocyte development
Sohn, Jiho; Natale, Joanne; Chew, Li-Jin et al.
2006In Journal of Neuroscience, 26 (38), p. 9722-9735
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Keywords :
gene profiling; cell lineage; CNP-EGFP mouse; cell cycle; myelin genes; cell differentiation
Abstract :
[en] Microarray analysis of oligodendrocyte lineage cells purified by fluorescence-activated cell sorting (FACS) from 2', 3'- cyclic nucleotide 3'-phosphodiesterase (CNP)-enhanced green fluorescent protein (EGFP) transgenic mice revealed Sox17 (SRY-box containing gene 17) gene expression to be coordinately regulated with that of four myelin genes during postnatal development. In CNP-EGFP-positive (CNP-EGFP(+)) cells, Sox17 mRNA and protein levels transiently increased between postnatal days 2 and 15, with white matter O4(+) preoligodendrocytes expressing greater Sox17 levels than Nkx2.2(+) ( NK2 transcription factor related, locus 2) NG2(+), or GalC(+) ( galactocerebroside) cells. In spinal cord, Sox17 protein expression was undetectable in the primary motor neuron domain between embryonic days 12.5 and 15.5 but was evident in Nkx2.2(+) and CCl+ cells. In cultured oligodendrocyte progenitor cells (OPCs), Sox17 levels were maximal in O4(+) cells and peaked during the phenotypic conversion from bipolar to multipolar. Parallel increases in Sox17 and p27 occurred before MBP protein expression, and Sox17 upregulation was prevented by conditions inhibiting differentiation. Sox17 down-regulation with small interfering RNAs increased OPC proliferation and decreased lineage progression after mitogen withdrawal, whereas Sox17 overexpression in the presence of mitogen had opposite effects. Sox17 overexpression enhanced myelin gene expression in OPCs and directly stimulated MBP gene promoter activity. These findings support important roles for Sox17 in controlling both oligodendrocyte progenitor cell cycle exit and differentiation.
Disciplines :
Neurosciences & behavior
Author, co-author :
Sohn, Jiho
Natale, Joanne
Chew, Li-Jin
Belachew, Shibeshih ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie
Cheng, Yi
Aguirre, Adan A.
Lytle, Judith
Nait-Oumesmar, Brahim
Kerninon, Christophe
Kanai-Azuma, Masami
Kanai, Yoshiakira
Gallo, Vittorio
Language :
English
Title :
Identification of Sox17 as a transcription factor that regulates oligodendrocyte development
Publication date :
20 September 2006
Journal title :
Journal of Neuroscience
ISSN :
0270-6474
eISSN :
1529-2401
Publisher :
Soc Neuroscience, Washington, United States - Washington
Volume :
26
Issue :
38
Pages :
9722-9735
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 January 2009

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