Reference : Postnatal NG2 proteoglycan-expressing progenitor cells are intrinsically multipotent ...
Scientific journals : Article
Human health sciences : Neurology
http://hdl.handle.net/2268/5032
Postnatal NG2 proteoglycan-expressing progenitor cells are intrinsically multipotent and generate functional neurons.
English
Belachew, Shibeshih mailto [Université de Liège - ULg > Département des sciences cliniques > Neurologie - CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Chittajallu, Ramesh [> > > >]
Aguirre, Adan A. [> > > >]
Yuan, Xiaoqing [> > > >]
Kirby, Martha [> > > >]
Anderson, Stacie [> > > >]
Gallo, Vittorio [> > > >]
2003
Journal of Cell Biology
Rockefeller University Press
161
1
169-86
Yes (verified by ORBi)
International
0021-9525
1540-8140
New York
NY
[en] 2',3'-Cyclic-Nucleotide Phosphodiesterases/genetics/metabolism ; Action Potentials/genetics ; Animals ; Animals, Newborn ; Antigens/genetics/metabolism ; Astrocytes/cytology/metabolism ; Cell Differentiation/genetics ; Cells, Cultured ; Dentate Gyrus/cytology/growth & development/metabolism ; Hippocampus/cytology/growth & development/metabolism ; Intermediate Filament Proteins/metabolism ; Mice ; Mice, Transgenic ; Models, Animal ; Multipotent Stem Cells/cytology/metabolism ; Nerve Tissue Proteins ; Neural Pathways/cytology/growth & development/metabolism ; Neurons/cytology/metabolism ; Oligodendroglia/cytology/metabolism ; Phenotype ; Promoter Regions, Genetic/genetics ; Proteoglycans/genetics/metabolism ; Recombinant Fusion Proteins/diagnostic use ; Spheroids, Cellular/cytology/metabolism ; gamma-Aminobutyric Acid/metabolism
[en] Neurogenesis is known to persist in the adult mammalian central nervous system (CNS). The identity of the cells that generate new neurons in the postnatal CNS has become a crucial but elusive issue. Using a transgenic mouse, we show that NG2 proteoglycan-positive progenitor cells that express the 2',3'-cyclic nucleotide 3'-phosphodiesterase gene display a multipotent phenotype in vitro and generate electrically excitable neurons, as well as astrocytes and oligodendrocytes. The fast kinetics and the high rate of multipotent fate of these NG2+ progenitors in vitro reflect an intrinsic property, rather than reprogramming. We demonstrate in the hippocampus in vivo that a sizeable fraction of postnatal NG2+ progenitor cells are proliferative precursors whose progeny appears to differentiate into GABAergic neurons capable of propagating action potentials and displaying functional synaptic inputs. These data show that at least a subpopulation of postnatal NG2-expressing cells are CNS multipotent precursors that may underlie adult hippocampal neurogenesis.
http://hdl.handle.net/2268/5032
10.1083/jcb.200210110

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