Reference : Expression of the green fluorescent protein in the oligodendrocyte lineage: a transgenic...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/5030
Expression of the green fluorescent protein in the oligodendrocyte lineage: a transgenic mouse for developmental and physiological studies.
English
Yuan, Xiaoqing [> > > >]
Chittajallu, Ramesh [> > > >]
Belachew, Shibeshih mailto [Université de Liège - ULg > Département des sciences cliniques > Neurologie - CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Anderson, Stacie [> > > >]
McBain, Chris J [> > > >]
Gallo, Vittorio [> > > >]
2002
Journal of Neuroscience Research
Wiley Interscience
70
4
529-45
Yes (verified by ORBi)
International
0360-4012
1097-4547
New York
NY
[en] 2',3'-Cyclic-Nucleotide Phosphodiesterases/genetics ; Animals ; Brain/cytology/embryology/metabolism ; Cell Division/drug effects ; Cell Lineage/physiology ; Cells, Cultured ; Flow Cytometry ; Green Fluorescent Proteins ; Immunohistochemistry ; Luminescent Proteins/biosynthesis/genetics ; Mice ; Mice, Transgenic ; Models, Animal ; Oligodendroglia/cytology/drug effects/metabolism ; Patch-Clamp Techniques ; Peripheral Nervous System/cytology/metabolism ; Potassium Channel Blockers/pharmacology ; Potassium Channels/drug effects/metabolism ; Promoter Regions, Genetic ; Schwann Cells/cytology/metabolism ; Stem Cells/cytology/drug effects/metabolism
[en] We generated a transgenic mouse expressing the enhanced green fluorescent protein (EGFP) under the control of the 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNP) promoter. EGFP(+) cells were visualized in live tissue throughout embryonic and postnatal development. Immunohistochemical analysis in brain tissue and in sciatic nerve demonstrated that EGFP expression was restricted to cells of the oligodendrocyte and Schwann cell lineages. EGFP was also strongly expressed in "adult" oligodendrocyte progenitors (OPs) and in gray matter oligodendrocytes. Fluorescence-activated cell sorting allowed high-yield purification of EGFP(+) oligodendrocyte-lineage cells from transgenic brains. Electrophysiological patch clamp recordings of EGFP(+) cells in situ demonstrated that OP cells displayed large outward tetraethylammonium (TEA)-sensitive K(+) currents and very small inward currents, whereas mature oligodendrocytes were characterized by expression of large inward currents and small outward K(+) currents. The proliferation rate of EGFP(+) cells in developing white matter decreased with the age of the animals and was strongly inhibited by TEA. Oligodendrocyte development and physiology can be studied in live tissue of CNP-EGFP transgenic mice, which represent a source of pure EGFP(+) oligodendrocyte-lineage cells throughout development.
http://hdl.handle.net/2268/5030
10.1002/jnr.10368
Copyright 2002 Wiley-Liss, Inc.

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Yuan JNR-2002.pdfPublisher postprint9.58 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.