Reference : Microscopic study of equine oocyte maturation within quaternary follicles.
Scientific congresses and symposiums : Unpublished conference
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/5020
Microscopic study of equine oocyte maturation within quaternary follicles.
English
[fr] ETude microscopique de la maturation de l'ovocyte dans le follicule quaternaire
Ponthier, Jérôme mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés >]
Beckers, Jean-François mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Deleuze, Stefan mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
19-Feb-2009
1
Yes
Yes
International
1st congress of the Balkan Network for Animal Reproduction Biotechnologies
18 février 2009 au 20 février 2009
Balkan Network for Animal Reproduction Biotechnologies
Sofia
Bulgarie
[en] Horse ; oocyte ; microscopy
[en] In various species a relationship between oocyte maturity and morphological characteristics of oocytes has been established. To our knowledge, immature oocyte aspect under light microscopy has been paid little attention in the equine. The aim of the study is to give a general description and analyse microscopic parameters of immature oocytes.
Follicles from abattoir ovaries are punctured after measurement of their diameter and their cumulus-oocyte complexes are recovered. Different characters such as oocyte diameter, cumulus aspect, granulosity and polarity of ooplasm are observed under light microscopy.
No correlation between estral activity, follicle maturity, cumulus cells aspect, polarity and granulosity of ooplasm was observed. Thickness of zona pellucida differs between oocytes with polar or non polar ooplasm. No correlation was observed between follicle diameter and oocyte diameter and none of the other studied parameters showed an influence on oocyte diameter.
Results show that no character of oocytes observed under light microscopy can be related to follicular origin or to any other character. Further studies have to be done to compare these immature oocytes with mature oocytes and to find the ultra-structural origin of characters observed under light microscopy.
Reproduction des Equidés et Carnivores domestiques
Researchers
http://hdl.handle.net/2268/5020

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