ORBi: Ponthier Jérôme - Microscopic study of equine oocyte maturation within quaternary follicles.

Reference : Microscopic study of equine oocyte maturation within quaternary follicles.


	Document type :	Scientific congresses and symposiums : Unpublished conference
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/5020

Title :	Microscopic study of equine oocyte maturation within quaternary follicles.
Language :	English
Alternative title :	[fr] ETude microscopique de la maturation de l'ovocyte dans le follicule quaternaire
Author, co-author :	Ponthier, Jérôme [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés >]
	Beckers, Jean-François [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
	Deleuze, Stefan [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Publication date :	19-Feb-2009
Page number :	1
Peer reviewed :	Yes
On invitation :	Yes
Audience :	International
Event name :	1st congress of the Balkan Network for Animal Reproduction Biotechnologies
Event date :	18 février 2009 au 20 février 2009
Event organizer :	Balkan Network for Animal Reproduction Biotechnologies
Event place (city) :	Sofia
Event country :	Bulgarie
Keywords :	[en] Horse ; oocyte ; microscopy
Abstract :	[en] In various species a relationship between oocyte maturity and morphological characteristics of oocytes has been established. To our knowledge, immature oocyte aspect under light microscopy has been paid little attention in the equine. The aim of the study is to give a general description and analyse microscopic parameters of immature oocytes. Follicles from abattoir ovaries are punctured after measurement of their diameter and their cumulus-oocyte complexes are recovered. Different characters such as oocyte diameter, cumulus aspect, granulosity and polarity of ooplasm are observed under light microscopy. No correlation between estral activity, follicle maturity, cumulus cells aspect, polarity and granulosity of ooplasm was observed. Thickness of zona pellucida differs between oocytes with polar or non polar ooplasm. No correlation was observed between follicle diameter and oocyte diameter and none of the other studied parameters showed an influence on oocyte diameter. Results show that no character of oocytes observed under light microscopy can be related to follicular origin or to any other character. Further studies have to be done to compare these immature oocytes with mature oocytes and to find the ultra-structural origin of characters observed under light microscopy.
Research units :	Reproduction des Equidés et Carnivores domestiques
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/5020

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