Article (Scientific journals)
Developmental Regulation of Neuroligand-Induced Responses in Cultured Oligodendroglia
Belachew, Shibeshih; Malgrange, Brigitte; Rigo, Jean-Michel et al.
1998In Neuroreport, 9 (6), p. 973-80
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Abstract :
[en] Using whole-cell patch-clamp techniques, we show that oligosphere-derived oligodendrocyte progenitor cells (OP) display GABA-, glutamate-, 5-HT-, glycine- and acetylcholine-gated inward currents. When OP differentiate into oligodendrocytes (ODC), the amplitude of peak currents elicited by saturating concentrations of these transmitters decreases except for 5-HT. Intracellular Ca2+ concentration changes induced by microperfusion of glutamate, 5-HT, TRH, met-enkephalin and substance P were monitored using a fluo-3-based calcium imaging system. When OP cells differentiate into ODC, a global decrease of the proportion of responding cells is observed. During type-2 astrocytes commitment, this proportion decreases for 5-HT, TRH- and metenkephalin stimulations whereas it remains constant for substance P and glutamate. These data demonstrate a development regulation of neurotransmitter- and neuropeptide-induced responses within the oligodendroglial lineage.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Belachew, Shibeshih ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie
Malgrange, Brigitte  ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Rigo, Jean-Michel;  Université de Liège - ULiège > CNCM
Rogister, Bernard  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Coucke, Paul;  Université de Liège - ULiège > Département des sciences cliniques > Radiothérapie
Mazy-Servais, Cécile;  Université de Liège - ULiège > CNCM
Moonen, Gustave  ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine
Language :
English
Title :
Developmental Regulation of Neuroligand-Induced Responses in Cultured Oligodendroglia
Publication date :
20 April 1998
Journal title :
Neuroreport
ISSN :
0959-4965
eISSN :
1473-558X
Publisher :
Lippincott Williams & Wilkins, United States
Volume :
9
Issue :
6
Pages :
973-80
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 January 2009

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