ORBi: De Seny Dominique - Monomeric calgranulins measured by SELDI-TOF mass spectrometry and calprotectin measured by ELISA as biomarkers in arthritis

Reference : Monomeric calgranulins measured by SELDI-TOF mass spectrometry and calprotectin measured...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Rheumatology Human health sciences : Gastroenterology & hepatology Human health sciences : Rheumatology Human health sciences : Gastroenterology & hepatology
	To cite this reference:	http://hdl.handle.net/2268/4957

Title :	Monomeric calgranulins measured by SELDI-TOF mass spectrometry and calprotectin measured by ELISA as biomarkers in arthritis
Language :	English
Author, co-author :	De Seny, Dominique [Centre Hospitalier Universitaire de Liège - CHU > > Rhumatologie >]
	Fillet, Marianne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Ribbens, Clio [Centre Hospitalier Universitaire de Liège - CHU > > Rhumatologie >]
	Marée, Raphaël [Université de Liège - ULg > > GIGA-Management : Plateforme bioinformatique >]
	Meuwis, Marie-Alice [Université de Liège - ULg > > GIGA-Management : Plate-forme protéomique >]
	Lutteri, Laurence [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
	Chapelle, Jean-Paul [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
	Wehenkel, Louis [Université de Liège - ULg > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation >]
	Louis, Edouard [Université de Liège - ULg > Département des sciences cliniques > Hépato-gastroentérologie - Relations académiques et scientifiques (Médecine) >]
	Merville, Marie-Paule [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
	Malaise, Michel [Centre Hospitalier Universitaire de Liège - CHU > > Rhumatologie >]
Publication date :	2008
Journal title :	Clinical Chemistry
Publisher :	American Association for Clinical Chemistry
Volume :	54
Pages :	1066-1075
Audience :	International
ISSN :	0009-9147
e-ISSN :	1530-8561
City :	Washington
Country :	DC
Keywords :	[en] Biological Markers ; Calgranulin A ; Calgranulin B ; Leukocyte L1 Antigen Complex ; S100 Proteins ; Serum Amyloid A Protein ; Arthritis, Rheumatoid ; Enzyme-Linked Immunosorbent Assay ; Psoriasis
Abstract :	[en] BACKGROUND: SELDI-TOF mass spectrometry (MS) is a high-throughput proteomic approach with potential for identifying novel forms of serum biomarkers of arthritis. METHODS: We used SELDI-TOF MS to analyze serum samples from patients with various forms of inflammatory arthritis. Several protein profiles were collected on different Bio-Rad Laboratories ProteinChip arrays (CM10 and IMAC-Cu(2+)) and were evaluated statistically to select potential biomarkers. RESULTS: SELDI-TOF MS analyses identified several calgranulin proteins [S100A8 (calgranulin A), S100A9 (calgranulin B), S100A9*, and S100A12 (calgranulin C)], serum amyloid A (SAA), SAA des-Arg (SAA-R), and SAA des-Arg/des-Ser (SAA-RS) as biomarkers and confirmed the results with other techniques, such as western blotting, immunoprecipitation, and nano-LC-MS/MS. The S100 proteins were all able to significantly differentiate samples from patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), and ankylosing spondylitis (AS) from those of patients with inflammatory bowel diseases used as an inflammatory control (IC) group, whereas the SAA, SAA-R, and SAA-RS proteins were not, with the exception of AS. The 4 S100 proteins were coproduced in all of the pathologies and were significantly correlated with the plasma calprotectin concentration; however, these S100 proteins were correlated with the SAA peak intensities only in the RA and IC patient groups. In RA, these S100 proteins (except for S100A12) were significantly correlated with the serum concentrations of C-reactive protein, matrix metalloproteinase 3, and anti-cyclic citrullinated peptide and with the Disease Activity Score (DAS(28)). CONCLUSIONS: The SELDI-TOF MS technology is a powerful approach for analyzing the status of monomeric, truncated, or posttranslationally modified forms of arthritis biomarkers, such as the S100A8, S100A9, S100A12, and SAA proteins. The fact that the SELDI-TOF MS data were correlated with results obtained with the classic calprotectin ELISA test supports the reliability of this new proteomic technique.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/94555

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