Article (Scientific journals)
Proteomics for prediction and characterization of response to infliximab in Crohn's disease: a pilot study.
Meuwis, Marie-Alice; Fillet, Marianne; Lutteri, Laurence et al.
2008In Clinical Biochemistry, 41 (12), p. 960-7
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Keywords :
Adult; Analysis of Variance; Anti-Inflammatory Agents/therapeutic use; Antibodies, Monoclonal/therapeutic use; Biological Markers/blood; CD40 Ligand/blood; Crohn Disease/blood/drug therapy; Enzyme-Linked Immunosorbent Assay; Female; Humans; Interleukin-6/blood; Male; Middle Aged; Multivariate Analysis; Pilot Projects; Platelet Factor 4/blood; Prognosis; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Abstract :
[en] OBJECTIVES: Infliximab is the first anti-TNFalpha accepted by the Food and Drug Administration for use in inflammatory bowel disease treatment. Few clinical, biological and genetic factors tend to predict response in Crohn's disease (CD) patient subcategories, none widely predicting response to infliximab. DESIGN AND METHODS: Twenty CD patients showing clinical response or non response to infliximab were used for serum proteomic profiling on Surface Enhanced Lazer Desorption Ionisation-Time of Flight-Mass Spectrometry (SELDI-TOF-MS), each before and after treatment. Univariate and multivariate data analysis were performed for prediction and characterization of response to infliximab. RESULTS: We obtained a model of classification predicting response to treatment and selected relevant potential biomarkers, among which platelet aggregation factor 4 (PF4). We quantified PF4, sCD40L and IL-6 by ELISA for correlation studies. CONCLUSIONS: This first proteomic pilot study on response to infliximab in CD suggests association between platelet metabolism and response to infliximab and requires validation studies on a larger cohort of patients.
Disciplines :
Gastroenterology & hepatology
Author, co-author :
Meuwis, Marie-Alice   ;  Université de Liège - ULiège > GIGA-Management : Plate-forme protéomique
Fillet, Marianne  ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Lutteri, Laurence ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Marée, Raphaël  ;  Université de Liège - ULiège > GIGA-Management : Plateforme bioinformatique
Geurts, Pierre ;  Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation
De Seny, Dominique ;  Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie
Malaise, Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie
Chapelle, Jean-Paul ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Wehenkel, Louis  ;  Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation
Belaiche, Jacques ;  Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie
Merville, Marie-Paule ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Louis, Edouard  ;  Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie - Relations académiques et scientifiques (Médecine)
 These authors have contributed equally to this work.
Language :
English
Title :
Proteomics for prediction and characterization of response to infliximab in Crohn's disease: a pilot study.
Publication date :
2008
Journal title :
Clinical Biochemistry
ISSN :
0009-9120
eISSN :
1873-2933
Publisher :
Elsevier Science, Tarrytown, United States - New York
Volume :
41
Issue :
12
Pages :
960-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 January 2009

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