Reference : Identification of factors that maintain mammalian outer hair cells in adult organ of ...
Scientific journals : Article
Human health sciences : Otolaryngology
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/4944
Identification of factors that maintain mammalian outer hair cells in adult organ of Corti explants
English
Malgrange, Brigitte mailto [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Rigo, Jean-Michel [Universiteit Hasselt - UH > > > >]
Coucke, Paul [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
Thiry, Marc mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anesthésie et réanimation >]
Hans, Grégory mailto [Université de Liège - ULg > Département des sciences cliniques > Neurologie >]
Nguyen, Laurent mailto [> > > >]
van de Water, Thomas R. [Université de Liège - ULg > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine]
Moonen, Gustave mailto [Centre Hospitalier Universitaire de Liège - CHU > > O.R.L. >]
Lefebvre, Philippe mailto [> > > >]
Aug-2002
Hearing Research
Elsevier Science Bv
170
1-2
48-58
Yes (verified by ORBi)
International
0378-5955
Amsterdam
[en] apoptosis ; organ of Corti ; outer hair cell ; trophic factor ; guinea pig
[en] Both outer hair cells (OHCs) and inner hair cells (IHCs) survive and mature in 3 days old rat organ of Corti explants cultured for I month in a minimal essential medium. In contrast. under the same culture conditions, only IHCs survive in explants from adult guinea pig organ of Corti while many of the OHCs are lost within the first 48 It. Hair cell Count,, show OHCs loss to be greater in the lower portion (i.e. middle turn) of the cochlea than Lit the apex. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) indicates that there is DNA damage in adult OHCs, within 8 h of explantation. Treatment of the adult organ of Corti explants with either actinomycin D (10(-7) M) or cycloheximide (10(-6) M) prevents most OHC losses . According to these results apoptosis may be the mechanism of OHC loss in adult organ of Corti explants, Stable membrane potentials recorded from the OHCs in both uncultured and actinomycin D-treated organ of Corti explants cultured for 72 h demonstrate the functional integrity of these hair cells. OHC losses in the adult guinea pig, organ of Corti cultures can also be prevented by treatment with several of the growth factors tested. i.e. acidic fibroblast growth factor (aFGF), insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), transforming growth factor-beta1 (TGF-beta1). and glial cell-derived neurotrophic factor (GDNF). The results of this study suggest that growth factor therapy may be applicable to the treatment of some hearing disorders. (C) 2002 Elsevier Science B.V. All rights reserved.
Researchers ; Professionals
http://hdl.handle.net/2268/4944

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