Article (Scientific journals)
Coupling of cell migration with neurogenesis by proneural bHLH factors.
Ge, Weihong; He, Fei; Kim, Kevin J. et al.
2006In Proceedings of the National Academy of Sciences of the United States of America, 103 (5), p. 1319-24
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Keywords :
Actins/chemistry; Animals; Basic Helix-Loop-Helix Transcription Factors/metabolism; Blotting, Western; Cell Differentiation; Cell Movement; Cerebral Cortex/pathology; Chromatin Immunoprecipitation; Cytoskeleton/metabolism; DNA/chemistry; DNA-Binding Proteins/chemistry/physiology; Down-Regulation; Electroporation; GTP Phosphohydrolases/metabolism; Gene Expression Regulation; Mice; Mice, Transgenic; Microscopy, Fluorescence; Microtubule-Associated Proteins/biosynthesis; Microtubules/metabolism; Mutation; Nerve Tissue Proteins/biosynthesis/metabolism; Neurons/metabolism; Neuropeptides/biosynthesis; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors/chemistry/physiology; Transfection; Up-Regulation; rhoA GTP-Binding Protein/metabolism
Abstract :
[en] After cell birth, almost all neurons in the mammalian central nervous system migrate. It is unclear whether and how cell migration is coupled with neurogenesis. Here we report that proneural basic helix-loop-helix (bHLH) transcription factors not only initiate neuronal differentiation but also potentiate cell migration. Mechanistically, proneural bHLH factors regulate the expression of genes critically involved in migration, including down-regulation of RhoA small GTPase and up-regulation of doublecortin and p35, which, in turn, modulate the actin and microtubule cytoskeleton assembly and enable newly generated neurons to migrate. In addition, we report that several DNA-binding-deficient proneural genes that fail to initiate neuronal differentiation still activate migration, whereas a different mutation of a proneural gene that causes a failure in initiating cell migration still leads to robust neuronal differentiation. Collectively, these data suggest that transcription programs for neurogenesis and migration are regulated by bHLH factors through partially distinct mechanisms.
Disciplines :
Biochemistry, biophysics & molecular biology
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Ge, Weihong
He, Fei
Kim, Kevin J.
Blanchi, Bruno
Coskun, Volkan
Nguyen, Laurent  ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie
Wu, Xiangbing
Zhao, Jing
Heng, Julian Ik-Tsen
Martinowich, Keri
Tao, Jifang
Wu, Hao
Castro, Diogo
Sobeih, Magdi M.
Corfas, Gabriel
Gleeson, Joseph G.
Greenberg, Michael E.
Guillemot, François
Sun, Yi E.
More authors (9 more) Less
Language :
English
Title :
Coupling of cell migration with neurogenesis by proneural bHLH factors.
Publication date :
2006
Journal title :
Proceedings of the National Academy of Sciences of the United States of America
ISSN :
0027-8424
eISSN :
1091-6490
Publisher :
National Academy of Sciences, Washington, United States - District of Columbia
Volume :
103
Issue :
5
Pages :
1319-24
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 January 2009

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