ORBi: Belachew Shibeshih - Glycine Triggers an Intracellular Calcium Influx in Oligodendrocyte Progenitor Cells Which Is Mediated by the Activation of Both the Ionotropic Glycine Receptor and Na+-Dependent Transporters

Reference : Glycine Triggers an Intracellular Calcium Influx in Oligodendrocyte Progenitor Cells Whi...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Anatomy (cytology, histology, embryology...) & physiology
	To cite this reference:	http://hdl.handle.net/2268/4915

Title :	Glycine Triggers an Intracellular Calcium Influx in Oligodendrocyte Progenitor Cells Which Is Mediated by the Activation of Both the Ionotropic Glycine Receptor and Na+-Dependent Transporters
Language :	English
Author, co-author :	Belachew, Shibeshih [Université de Liège - ULg > Département des sciences cliniques > Neurologie >]
	Malgrange, Brigitte [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
	Rigo, Jean-Michel [Universiteit Hasselt - UH > > > >]
	Rogister, Bernard [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
	Leprince, Pierre [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
	Hans, Grégory [Centre Hospitalier Universitaire de Liège - CHU > > Anesthésie et réanimation >]
	Nguyen, Laurent [Université de Liège - ULg > Département des sciences cliniques > Neurologie >]
	Moonen, Gustave [Université de Liège - ULg > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine]
Publication date :	Jun-2000
Journal title :	European Journal of Neuroscience
Volume :	12
Issue/season :	6
Pages :	1924-30
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0953-816X
Keywords :	[en] Oligodendrocyte ; Neurotransmitter ; patch-clamp
Abstract :	[en] Using fluo-3 calcium imaging, we demonstrate that glycine induces an increase in intracellular calcium concentration ([Ca2+]i) in cortical oligodendrocyte progenitor (OP) cells. This effect results from a calcium entry through voltage-gated calcium channels (VGCC), as it is observed only in OP cells expressing such channels, and it is abolished either by removal of calcium from the extracellular medium or by application of an L-type VGCC blocker. Glycine-triggered Ca2+ influx in OP cells actually results from an initial depolarization that is the consequence of the activation of both the ionotropic glycine receptor (GlyR) and Na+-dependent transporters, most probably the glycine transporters 1 (GLYT1) and/or 2 (GLYT2) which are colocalized in these cells. Through this GlyR- and transporter-mediated effect on OP intrcellular calcium concentration [Ca2+]i, glycine released by neurons may, as well as other neurotransmitters, serve as a signal between neurons and OP during development.
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Target :	Researchers ; Students
Permalink :	http://hdl.handle.net/2268/4915

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