Article (Scientific journals)
Functional glycine receptors are expressed by postnatal nestin-positive neural stem/progenitor cells
Nguyen, Laurent; Malgrange, Brigitte; Belachew, Shibeshih et al.
2002In European Journal of Neuroscience, 15 (8), p. 1299-1305
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Keywords :
glycine receptor; immunocytochemistry; neural stem; precursor cells; rat striatum; RT-PCR; whole-cell patch-clamp
Abstract :
[en] Multipotent neural stem and progenitor cells (NS/PCs) are well-established cell subpopulations occurring in the developing, and also in the mature mammalian nervous systems. Trophic and transcription factors are currently the main signals known to influence the development and the commitment of NS/PCs and their progeny. However, recent studies suggest that neurotransmitters could also contribute to neural development. In that respect, rodent-cultured embryonic NS/PCs have been reported to express functional neurotransmitter receptors. No similar investigation has, however, been made in postnatal and/or in adult rodent brain stem cells. In this study, using RT-PCR and immunocytochemical methods, we show that alpha(1) -, alpha(2) - and beta-subunit mRNAs and alpha-subunit proteins of the glycine ionotropic receptor are expressed by 80.5 +/- 0.9% of postnatal rat striatum-derived, nestin-positive cells within cultured neurospheres. Whole-cell patch-clamp experiments further demonstrated that glycine triggers in 33.5% of these cells currents that can be reversibly blocked by strychnine and picrotoxin. This demonstrates that NS/PCs express functional glycine receptors, the consequence(s) of their activation remaining unknown.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Nguyen, Laurent  ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie
Malgrange, Brigitte  ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Belachew, Shibeshih ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie
Rogister, Bernard  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Rocher, Véronique
Moonen, Gustave  ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine
Rigo, Jean-Michel;  Universiteit Hasselt - UH
Language :
English
Title :
Functional glycine receptors are expressed by postnatal nestin-positive neural stem/progenitor cells
Publication date :
April 2002
Journal title :
European Journal of Neuroscience
ISSN :
0953-816X
eISSN :
1460-9568
Publisher :
Blackwell Publishing Ltd, Oxford, United Kingdom
Volume :
15
Issue :
8
Pages :
1299-1305
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 January 2009

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