p63 is a prostate basal cell marker and is required for prostate development.

Adenocarcinoma/metabolism/pathology; Animals; Biological Markers; Cell Nucleus/metabolism; Cells, Cultured; DNA-Binding Proteins; Epithelial Cells/metabolism; Genes, Tumor Suppressor; Humans; Male; Membrane Proteins; Mice; Mice, Inbred Strains; Neoplasm Invasiveness; Phosphoproteins/metabolism/physiology; Prostate/cytology/growth & development/metabolism; Prostatic Intraepithelial Neoplasia/metabolism/pathology; Prostatic Neoplasms/metabolism/pathology; Protein Isoforms/metabolism; Reference Values; Trans-Activators; Tumor Suppressor Proteins

Abstract :

[en] The p53 homologue p63 encodes for different isotypes able to either transactivate p53 reporter genes (TAp63) or act as p53-dominant-negatives (DeltaNp63). p63 is expressed in the basal cells of many epithelial organs and its germline inactivation in the mouse results in agenesis of organs such as skin appendages and the breast. Here, we show that prostate basal cells, but not secretory or neuroendocrine cells, express p63. In addition, prostate basal cells in culture predominantly express the DeltaNp63alpha isotype. In contrast, p63 protein is not detected in human prostate adenocarcinomas. Finally, and most importantly, p63(-/-) mice do not develop the prostate. These results indicate that p63 is required for prostate development and support the hypothesis that basal cells represent and/or include prostate stem cells. Furthermore, our results show that p63 immunohistochemistry may be a valuable tool in the differential diagnosis of benign versus malignant prostatic lesions.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology
Urology & nephrology

Author, co-author :

Signoretti, Sabina

Waltregny, David ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases - Département des sciences biomédicales et précliniques

Dilks, James

Isaac, Beth

Lin, Douglas

Garraway, Levi

Yang, Annie

Montironi, Rondolfo

McKeon, Frank

Loda, Massimo

Language :

English

Title :

p63 is a prostate basal cell marker and is required for prostate development.

Publication date :

2000

Journal title :

American Journal of Pathology

ISSN :

0002-9440

eISSN :

1525-2191

Publisher :

American Society for Investigative Pathology, Bethesda, United States - Maryland

Volume :

157

Issue :

Pages :

1769-75

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 January 2009

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507

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477

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434

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