Reference : Loss of type IV collagen alpha 5 and alpha 6 chains in human invasive prostate carcinomas
Scientific journals : Article
Human health sciences : Urology & nephrology
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/4718
Loss of type IV collagen alpha 5 and alpha 6 chains in human invasive prostate carcinomas
English
Dehan, Pierre mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Waltregny, David mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases - Département des sciences biomédicales et précliniques >]
Beschin, Alain [ > > ]
Noël, Agnès mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme >]
Castronovo, Vincenzo mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - Labo de recherche sur les métastases >]
Tryggvason, Karl [> > > >]
De Leval, Jean mailto [Centre Hospitalier Universitaire de Liège - CHU > > Urologie >]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]
1997
American Journal of Pathology
American Society for Investigative Pathology
151
4
1097-104
Yes (verified by ORBi)
International
0002-9440
1525-2191
Bethesda
MD
[en] Adenocarcinoma/metabolism/pathology ; Animals ; Antibodies, Monoclonal ; Antibody Specificity ; Basement Membrane/metabolism ; Collagen/immunology/metabolism ; Humans ; Immunoblotting ; Immunohistochemistry ; Male ; Mice ; Mice, Inbred BALB C ; Neoplasm Invasiveness ; Prostate/metabolism ; Prostatic Intraepithelial Neoplasia/metabolism/pathology ; Prostatic Neoplasms/metabolism/pathology
[en] Type IV collagen, a major component of basement membranes, is organized in a network responsible for the mechanical resistance of the basement membranes. It also plays a key role in epithelial cell adhesion to basement membranes. This study was designed to investigate the distribution of type IV collagen alpha-chains in normal, preneoplastic, and malignant prostate basement membranes. For this purpose, immunohistochemistry using specific antibodies raised against the different alpha-chains of type IV collagen was performed in eight normal samples, six prostatic intraepithelial neoplasia, and 20 malignant lesions of the prostate. Our results demonstrate the presence of the "novel" alpha 5 (IV) and alpha 6 (IV) chains along with the "classical" alpha 1 (IV)/alpha 2 (IV) chains in the basement membrane of the normal prostate gland. The alpha 3 (IV) chain was never detected in any prostate specimen. Prostatic intraepithelial neoplasia showed a similar immunostaining pattern to that found in normal glands. In cancer gland basement membranes, we demonstrate for the first time a specific loss of the alpha 5 (IV) and alpha 6 (IV) chains, whereas the classical alpha 1 (IV) and alpha 2 (IV) chains were consistently exhibited. Additionally, type VII collagen colocalized with alpha 5 (IV) collagen chain, and these two proteins, which were always observed in normal and prostatic intraepithelial neoplasia gland basement membranes, were lost in invasive carcinoma basement membranes. This observation raises questions about the possible association or cooperation between alpha 5 (IV)/alpha 6 (IV) chains and anchoring fibrils in prostate glands basement membrane.
Researchers ; Professionals
http://hdl.handle.net/2268/4718

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