Article (Scientific journals)
Identification of specific reachable molecular targets in human breast cancer using a versatile ex vivo proteomic method
Castronovo, Vincenzo; Kischel, Philippe; Guillonneau, Francois et al.
2007In Proteomics, 7 (8), p. 1188-1196
Peer Reviewed verified by ORBi
 

Files


Full Text
Pub#51 Proteomics 2007.pdf
Publisher postprint (484.97 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
biomarker discovery; biotinylation; breast cancer; mass spectrometry; tumor targeting
Abstract :
[en] Targeting of tumoral tissues is one of the most promising approaches to improve both the efficacy and safety of anticancer treatments. The identification of valid targets, including proteins specifically and abundantly expressed in cancer lesions, is of utmost importance. Despite state-of-the-art technologies, the discovery of cancer-associated target proteins still faces the limitation, in human tissues, of antigen accessibility to suitable high-affinity ligands such as human mAb bound to bioactive molecules. Terminal perfusion of tumor-bearing mice or ex vivo perfusion of human cancer-bearing organs with a reactive biotin ester solution has successfully led to the identification of novel accessible biomarkers. This methodology is however restricted to perfusable organs, and excludes most of the tissues of interest to targeted therapies, e.g. primary breast cancer and metastases. Herein, we report on the development of a new chemical proteomic method that bypasses the perfusion step and thus offers the potential to identify accessible molecular targets in virtually all types of animal and human tissues. We have validated our new procedure by identifying biomarkers selectively expressed in human breast carcinoma. Overall, this powerful technology may lay the ground not only for custom-made therapies in cancer, but also for the development of therapies that need to be selectively delivered in a specific tissue.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Castronovo, Vincenzo ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire
Kischel, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases
Guillonneau, Francois
de Leval, Laurence ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Defechereux, Thierry ;  Centre Hospitalier Universitaire de Liège - CHU > Chirurgie abdominale- endocrinienne et de transplantation
De Pauw, Edwin  ;  Université de Liège - ULiège > Chimie physique, spectrométrie de masse
Neri, Dario
Waltregny, David  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases
Language :
English
Title :
Identification of specific reachable molecular targets in human breast cancer using a versatile ex vivo proteomic method
Publication date :
April 2007
Journal title :
Proteomics
ISSN :
1615-9853
eISSN :
1615-9861
Publisher :
Wiley-V C H Verlag Gmbh, Weinheim, Germany
Volume :
7
Issue :
8
Pages :
1188-1196
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 January 2009

Statistics


Number of views
98 (8 by ULiège)
Number of downloads
2 (1 by ULiège)

Scopus citations®
 
27
Scopus citations®
without self-citations
15
OpenCitations
 
28

Bibliography


Similar publications



Contact ORBi