Functional and profiling studies prove that prostate cancer upregulated neuroblastoma thymosin beta is the true human homologue of rat thymosin beta 15
[en] A peptide with a sequence identical to rat thymosin beta(Tb)15 was reported to be upregulated in human prostate cancer. However, in this report we provide evidence that TbNB, initially identified in human neuroblastoma, is the only Tb isoform upregulated in human prostate cancer and that the Tb15 sequence is not present herein. In addition, we demonstrate that human TbNB has a higher affinity for actin in comparison to Tb4 and promotes cell migration. In combination, this experimentally validates TbNB as functional homologue of rat Tb15 in the human organism and clarifies the current composition of the human Tb family. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Dhaese, S.
Jonckheere, V.
Goethals, M.
Waltregny, David ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases
Vandekerckhove, J.
Ampe, C.
Van Troys, M.
Language :
English
Title :
Functional and profiling studies prove that prostate cancer upregulated neuroblastoma thymosin beta is the true human homologue of rat thymosin beta 15
Wang W.S., Chen P.M., Hsiao H.L., Ju S.Y., and Su Y. Overexpression of the thymosin beta-4 gene is associated with malignant progression of SW480 colon cancer cells. Oncogene 22 (2003) 3297-3306
Lee S.H., Son M.J., Oh S.H., Rho S.B., Park K., Kim Y.J., Park M.S., and Lee J.H. Thymosin {beta}(10) inhibits angiogenesis and tumor growth by interfering with Ras function. Cancer Res. 65 (2005) 137-148
Bao L., Loda M., Janmey P.A., Stewart R., Anand-Apte B., and Zetter B.R. Thymosin beta 15: a novel regulator of tumor cell motility upregulated in metastatic prostate cancer. Nat. Med. 2 (1996) 1322-1328
Huff T., Muller C.S., Otto A.M., Netzker R., and Hannappel E. β-Thymosins, small acidic peptides with multiple functions. Int. J. Biochem. Cell Biol. 33 (2001) 205-220
Yokoyama M., Nishi Y., Yoshii J., Okubo K., and Matsubara K. Identification and cloning of neuroblastoma-specific and nerve tissue-specific genes through compiled expression profiles. DNA Res. 3 (1996) 311-320
Hutchinson L.M., et al. Development of a sensitive and specific enzyme-linked immunosorbent assay for thymosin beta15, a urinary biomarker of human prostate cancer. Clin. Biochem. 38 (2005) 558-571
Choe J., Sun W., Yoon S.Y., Rhyu I.J., Kim E.H., and Kim H. Effect of thymosin beta15 on the branching of developing neurons. Biochem. Biophys. Res. Commun. 331 (2005) 43-49
Choi S.Y., Kim D.K., Eun B., Kim K., Sun W., and Kim H. Anti-apoptotic function of thymosin-beta in developing chick spinal motoneurons. Biochem. Biophys. Res. Commun. 346 (2006) 872-878
Koutrafouri V., Leondiadis L., Ferderigos N., Avgoustakis K., Livaniou E., Evangelatos G.P., and Ithakissios D.S. Synthesis and angiogenetic activity in the chick chorioallantoic membrane model of thymosin beta-15. Peptides 24 (2003) 107-115
Spudich J.A., and Watt S. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J. Biol. Chem. 246 (1971) 4866-4871
Brenner S.L., and Korn E.D. On the mechanism of actin monomer-polymer subunit exchange at steady state. J. Biol. Chem. 258 (1983) 5013-5020
Dassesse T., de Leval X., de Leval L., Pirotte B., Castronovo V., and Waltregny D. Activation of the thromboxane A2 pathway in human prostate cancer correlates with tumor Gleason score and pathologic stage. Eur. Urol. 50 (2006) 1021-1031 discussion 1031
Van Troys M., Dewitte D., Goethals M., Carlier M.F., Vandekerckhove J., and Ampe C. The actin binding site of thymosin beta 4 mapped by mutational analysis. Embo J. 15 (1996) 201-210
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., and Vandekerckhove J. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nat. Biotechnol. 21 (2003) 566-569
Schmidt M., Schler G., Gruensfelder P., and Hoppe F. Differential gene expression in a paclitaxel-resistant clone of a head and neck cancer cell line. Eur. Arch. Otorhinolaryngol. 263 (2006) 127-134
Bao L., Loda M., and Zetter B.R. Thymosin beta15 expression in tumor cell lines with varying metastatic potential. Clin. Exp. Meta. 16 (1998) 227-233
Otto A.M., Muller C.S., Huff T., and Hannappel E. Chemotherapeutic drugs change actin skeleton organization and the expression of beta-thymosins in human breast cancer cells. J. Cancer Res. Clin. Oncol. 128 (2002) 247-256
Banyard J., Hutchinson L.M., and Zetter B.R. Thymosin {beta}-NB is the human isoform of rat thymosin {beta}15. Ann. NY Acad. Sci. (2007)
Safer D., Sosnick T.R., and Elzinga M. Thymosin beta 4 binds actin in an extended conformation and contacts both the barbed and pointed ends. Biochemistry 36 (1997) 5806-5816
Domanski M., Hertzog M., Coutant J., Gutsche-Perelroizen I., Bontems F., Carlier M.F., Guittet E., and van Heijenoort C. Coupling of folding and binding of thymosin beta4 upon interaction with monomeric actin monitored by nuclear magnetic resonance. J. Biol. Chem. 279 (2004) 23637-23645
Irobi E., Aguda A.H., Larsson M., Guerin C., Yin H.L., Burtnick L.D., Blanchoin L., and Robinson R.C. Structural basis of actin sequestration by thymosin-beta4: implications for WH2 proteins. Embo J. 23 (2004) 3599-3608
Vancompernolle K., Goethals M., Huet C., Louvard D., and Vandekerckhove J. G- to F-actin modulation by a single amino acid substitution in the actin binding site of actobindin and thymosin beta 4. Embo J. 11 (1992) 4739-4746
Huff T., Muller C.S., and Hannappel E. C-terminal truncation of thymosin beta10 by an intracellular protease and its influence on the interaction with G-actin studied by ultrafiltration. FEBS Lett. 414 (1997) 39-44
Eadie J.S., Kim S.W., Allen P.G., Hutchinson L.M., Kantor J.D., and Zetter B.R. C-terminal variations in beta-thymosin family members specify functional differences in actin-binding properties. J. Cell Biochem. 77 (2000) 277-287
Chakravatri A., et al. Thymosin beta-15 predicts for distant failure in patients with clinically localized prostate cancer-results from a pilot study. Urology 55 (2000) 635-638