Reference : A chemical proteomics approach for the identification of accessible antigens expresse...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Human health sciences : Oncology
Human health sciences : Urology & nephrology
http://hdl.handle.net/2268/4697
A chemical proteomics approach for the identification of accessible antigens expressed in human kidney cancer
English
Castronovo, Vincenzo mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire >]
Waltregny, David mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases >]
Kischel, Philippe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases >]
Roesli, Cristof [ETH Zurich > Institute of Pharmaceutical Sciences]
Elia, Giuliano [ETH Zurich > Institute of Pharmaceutical Sciences]
Rybak, Jascha N. [ETH Zurich > Institute of Pharmaceutical Sciences]
Neri, Dario [ETH Zurich > Institute of Pharmaceutical Sciences]
Nov-2006
Molecular & Cellular Proteomics
Amer Soc Biochemistry Molecular Biology Inc
5
11
2083-2091
Yes (verified by ORBi)
International
1535-9476
Bethesda
[en] A promising avenue toward the development of more selective anticancer drugs consists in the targeted delivery of bioactive molecules to the tumor environment by means of binding molecules specific to tumor-associated markers. We have used a chemical proteomics approach based on the ex vivo perfusion and biotinylation of accessible structures within surgically resected human kidneys with tumor to gain information about accessible and abundant antigens that are overexpressed in human cancer. This procedure led to the selective labeling with biotin of vascular structures. Biotinylated proteins were purified on streptavidin resin and identified using mass spectrometric methodologies, revealing 637 proteins, 184 of which were only found in tumor specimens and 223 of which were only found in portions of normal kidneys. Immunohistochemical and PCR analysis confirmed that several of the putative cancer antigens identified in this study are indeed preferentially expressed in tumors. In conclusion, we have developed a methodology that allows the identification of accessible biomarkers in human tissues. The tumor-associated antigens identified in this study may be suitable targets for antibody-based anticancer therapies. The experimental approach described here should be applicable to other surgical specimens and to other pathologies as well as to the study of basic physiological and immunological processes.
http://hdl.handle.net/2268/4697
10.1074/mcp.M600164-MCP200

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