Article (Scientific journals)
A chemical proteomics approach for the identification of accessible antigens expressed in human kidney cancer
Castronovo, Vincenzo; Waltregny, David; Kischel, Philippe et al.
2006In Molecular and Cellular Proteomics, 5 (11), p. 2083-2091
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Abstract :
[en] A promising avenue toward the development of more selective anticancer drugs consists in the targeted delivery of bioactive molecules to the tumor environment by means of binding molecules specific to tumor-associated markers. We have used a chemical proteomics approach based on the ex vivo perfusion and biotinylation of accessible structures within surgically resected human kidneys with tumor to gain information about accessible and abundant antigens that are overexpressed in human cancer. This procedure led to the selective labeling with biotin of vascular structures. Biotinylated proteins were purified on streptavidin resin and identified using mass spectrometric methodologies, revealing 637 proteins, 184 of which were only found in tumor specimens and 223 of which were only found in portions of normal kidneys. Immunohistochemical and PCR analysis confirmed that several of the putative cancer antigens identified in this study are indeed preferentially expressed in tumors. In conclusion, we have developed a methodology that allows the identification of accessible biomarkers in human tissues. The tumor-associated antigens identified in this study may be suitable targets for antibody-based anticancer therapies. The experimental approach described here should be applicable to other surgical specimens and to other pathologies as well as to the study of basic physiological and immunological processes.
Disciplines :
Biochemistry, biophysics & molecular biology
Oncology
Urology & nephrology
Author, co-author :
Castronovo, Vincenzo ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire
Waltregny, David  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases
Kischel, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases
Roesli, Cristof;  ETH Zurich > Institute of Pharmaceutical Sciences
Elia, Giuliano;  ETH Zurich > Institute of Pharmaceutical Sciences
Rybak, Jascha N.;  ETH Zurich > Institute of Pharmaceutical Sciences
Neri, Dario;  ETH Zurich > Institute of Pharmaceutical Sciences
Language :
English
Title :
A chemical proteomics approach for the identification of accessible antigens expressed in human kidney cancer
Publication date :
November 2006
Journal title :
Molecular and Cellular Proteomics
ISSN :
1535-9476
eISSN :
1535-9484
Publisher :
Amer Soc Biochemistry Molecular Biology Inc, Bethesda, United States - Maryland
Volume :
5
Issue :
11
Pages :
2083-2091
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 January 2009

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