Reference : Synthesis of [Gamma-32p]Thiamine Triphosphate

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/4656

Title :	Synthesis of [Gamma-32p]Thiamine Triphosphate
Language :	English
Author, co-author :	Grandfils, Christian [Université de Liège - ULg > > Biochimie et physiologie générale >]
	Bettendorff, Lucien [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
	de Rycker, C. [> > > >]
	Schoffeniels, Ernest [> > > >]
Publication date :	1988
Journal title :	Analytical Biochemistry
Volume :	169
Issue/season :	2
Pages :	274-278
Audience :	International
ISSN :	0003-2697
Abstract :	[en] We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange purification steps, the thiamine derivative has a specific radioactivity of 10 Ci/mmol. The average final yield synthesis is about 10%.
Permalink :	http://hdl.handle.net/2268/4656

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