Reference : Detection of Human Papillomaviruses in Paraffin-Embedded Biopsies of Cervical Intraepith...
Scientific journals : Article
Human health sciences : Dermatology
http://hdl.handle.net/2268/4555
Detection of Human Papillomaviruses in Paraffin-Embedded Biopsies of Cervical Intraepithelial Lesions: Analysis by Immunohistochemistry, in Situ Hybridization, and the Polymerase Chain Reaction
English
Delvenne, Philippe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Fontaine, Marie-Anne [> > > >]
Delvenne, Catherine mailto [> > > >]
Nikkels, Arjen mailto [Université de Liège - ULg > > Dermatologie >]
Boniver, Jacques mailto [Université de Liège - ULg > > Anatomie pathologique >]
Jan-1994
Modern Pathology : An Official Journal of the United States & Canadian Academy of Pathology, Inc
7
1
113-119
Yes (verified by ORBi)
International
0893-3952
[en] One hundred and forty biopsies with an initial diagnosis of cervical intraepithelial lesion (CIL) were tested for the presence of human papillomavirus (HPV) by immunohistochemistry and in situ hybridization using commercial biotinylated probes (Vira-Type in situ assay; Digene Diagnostics, Silver Spring, MD) or probes labeled with digoxigenin by the random primer technique. Immunohistochemistry was more inferior to the in situ hybridization method, with a detection rate of 14% (20/140) compared to 61% (86/140) for the in situ assay with the digoxigenin-labeled probes. Biotinylated probes proved to be slightly less sensitive than digoxigenin-labeled probes, with a detection rate of 53% (74/140). Although less sensitive in our series taken as a whole, immunohistochemistry was positive in a few cases of CILs negative by in situ hybridization, so that the association of these techniques gave the highest detection rate (66%; 92/140). The CILs that remained negative with these methods (34%; 48/140) were investigated by the polymerase chain reaction (PCR) using consensus primers to determine definitively the presence of HPV in these lesions and were reviewed histologically to assess the diagnosis of CILs. The PCR method increased the detection rate of HPV in our series to 76% (107/140). The diagnosis of CILs was confirmed for all the biopsy specimens positive by PCR (15/15; 100%) and for all the HPV negative tissues with histological features of a high-grade lesions (7/7; 100%).(ABSTRACT TRUNCATED AT 250 WORDS)
http://hdl.handle.net/2268/4555

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