[en] The present study focuses on the impact of microbial activities on the performance of various long-term operated laboratory-scale permeable reactive barriers. The barriers contained both aquifer and Fe-0 compartments and had received either sulfate or iron(Ill)-EDTAto promote sulfatereducing and iron(Ill)-reducing bacteria, respectively. After dismantlement of the compartments after almost 3 years of operation, DNA-based PCR-DGGE analysis revealed the presence of methanogenic, sulfate-reducing, metalreducing, and denitrifying bacteria within as well as up- and downgradient of the Fe-0 matrix. Under all imposed conditions, the main secondary phases were vivianite, siderite, ferrous hydroxy carbonate, and carbonate green rust as found by scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX), and X-ray diffraction (XRD). Under sulfate-reduction promoting conditions, iron sulfides were formed in addition, resulting in 7 and 10 times higher degradation rates for PCE and TICE, respectively, compared to unreacted iron. These results indicate that the presence of sulfate-reducing bacteria in or around iron barriers and the subsequent formation of iron sulfides might increase the barrier reactivity.