ORBi: Deumens R. - Olfactory ensheathing cells, olfactory nerve fibroblasts and biomatrices to promote long-distance axon regrowth and functional recovery in the dorsally hemisected adult rat spinal cord

Reference : Olfactory ensheathing cells, olfactory nerve fibroblasts and biomatrices to promote long...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Neurology Engineering, computing & technology : Materials science & engineering Physical, chemical, mathematical & earth Sciences : Chemistry
	To cite this reference:	http://hdl.handle.net/2268/4376

Title :	Olfactory ensheathing cells, olfactory nerve fibroblasts and biomatrices to promote long-distance axon regrowth and functional recovery in the dorsally hemisected adult rat spinal cord
Language :	English
Author, co-author :	Deumens, R. [University of Maastricht > Department of Psychiatry and Neuropsychology > Division Neuroscience > >]
	Koopmans, G. C. [University of Maastricht > Department of Psychiatry and Neuropsychology > Division Neuroscience > >]
	Honig, W. M. M. [Academic Hospital Maastricht > Department of Anesthesiology > > >]
	Hamers, F. P. T. [University Medical Center Utrecht, Rudolf Magnus Institute for Neuroscience > Department of Physical Medicine and Rehabilitation > > >]
	Maquet, Véronique [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > > >]
	Jérôme, Robert [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
	Steinbusch, H. W. M. [University of Maastricht > Department of Psychiatry and Neuropsychology > Division Neuroscience > >]
	Joosten, E. A. J. [Academic Hospital Maastricht > Department of Anesthesiology > > >]
Publication date :	Jul-2006
Journal title :	Experimental Neurology
Publisher :	Academic Press Inc Elsevier Science
Volume :	200
Issue/season :	1
Pages :	89-103
Audience :	International
ISSN :	0014-4886
City :	San Diego
Keywords :	[en] biomaterial ; scaffold
Abstract :	[en] Cellular transplantation, including olfactory ensheathing cells (OEC) and olfactory nerve fibroblasts (ONF), after experimental spinal cord injury in the rat has previously resulted in regrowth of severed corticospinal (CS) axons across small lesion gaps and partial functional recovery. In order to stimulate CS axon regrowth across large lesion gaps, we used a multifactorial transplantation strategy to create an OEC/ONF continuum in spinal cords with a 2-mm-long dorsal hemisection lesion gap. This strategy involved the use of aligned OEG/ONF-poly(D,L)-lactide biomatrix bridges within the lesion gap and OEC/ONF injections at I mm rostral and caudal to the lesion gap. In order to test the effects of this complete strategy, control animals only received injections with culture medium rostral and caudal to the lesion gap. Anatomically, our multifactorial intervention resulted in an enhanced presence of injured CS axons directly rostral to the lesion gap (65.0 +/- 12.8% in transplanted animals versus 13.1 +/- 3.9% in control animals). No regrowth of these axons was observed through the lesion site, which may be related to a lack of OEC/ONF survival on the biomatrices. Furthermore, a 10-fold increase of neurofilament-positive axon ingrowth into the lesion site as compared to untreated control animals was observed. With the use of quantitative gait analysis, a modest recovery in stride length and swing speed of the hind limbs was observed. Although multifactorial strategies may be needed to stimulate repair of large spinal lesion gaps, we conclude that the combined use of OEC/ONF and poly(D,L)-lactide biomatrices is rather limited.
Research units :	Center for Education and Research on Macromolecules (CERM)
Funders :	the International Spinal Research Trust (ISRT; STR057 to E.A.J.J.)
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/4376
DOI :	10.1016/j.expneurol.2006.01.030
Other URL :	http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WFG-4JF8HKD-1-3&_cdi=6794&_user=532038&_orig=browse&_coverDate=07%2F31%2F2006&_sk=997999998&view=c&wchp=dGLbVlb-zSkzV&md5=5853d834ae8d26faadea096e9ddbd5cf&ie=/sdarticle.pdf
Commentary :	The authors acknowledge Experimental Neurology (Wiley) for allowing them to archive this paper.

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