ORBi: Dossou Katina Sourou Sylvestre - Optimization of the LC enantioseparation of chiral pharmaceuticals using cellulose tris(4-chloro-3-methylphenylcarbamate) as chiral selector and polar non-aqueous mobile phases

Reference : Optimization of the LC enantioseparation of chiral pharmaceuticals using cellulose tris(...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Pharmacy, pharmacology & toxicology
	To cite this reference:	http://hdl.handle.net/2268/41528

Title :	Optimization of the LC enantioseparation of chiral pharmaceuticals using cellulose tris(4-chloro-3-methylphenylcarbamate) as chiral selector and polar non-aqueous mobile phases
Language :	English
Author, co-author :	Dossou, Katina Sourou Sylvestre [Université de Liège - ULg > > > Doct. sc. bioméd. & pharma. (Bologne)]
	Chiap, Patrice [Université de Liège - ULg > > Pharmacologie clinique >]
	Chankvetadze, Bezhan [University of Munster > > > >]
	Servais, Anne-Catherine [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Fillet, Marianne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Crommen, Jacques [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Publication date :	2010
Journal title :	Journal of Separation Science
Publisher :	Wiley-VCH Verlag Gmbh
Volume :	33
Pages :	1699-1707
Audience :	International
ISSN :	1615-9306
e-ISSN :	1615-9314
City :	Weinheim
Country :	Germany
Keywords :	[en] Chiral separations ; Experimental design ; Optimisation ; Polar organic solvent chromatography ; Polysaccharide-based chiral stationary phase
Abstract :	[en] The resolving power of a new commercial polysaccharide-based chiral stationary phase, Sepapak-4, with cellulose tris(4-chloro-3-methylphenylcarbamate) coated on silica microparticles as chiral selector, was evaluated toward the enantioseparation of ten basic drugs with widely different structures and hydrophobic properties, using ACN as the main component of the mobile phase. A multivariate approach (experimental design) was used to screen the factors (temperature, n-hexane content, acidic and basic additives) likely to influence enantioresolution. Then, the optimization was performed using a face-centered central composite design. Complete enantioseparation could be obtained for almost all tested chiral compounds, demonstrating the high chiral discrimination ability of this chiral stationary phase using polar organic mobile phases made up of ACN and containing an acidic additive (TFA or formic acid), 0.1% diethylamine and n-hexane. These results clearly illustrate the key role of the nature of the acidic additive in the mobile phase.
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Politique Scientifique Fédérale (Belgique) = Belgian Federal Science Policy
Permalink :	http://hdl.handle.net/2268/41528

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