[en] In the course of varicella-zoster virus (VZV) infection, some viral capsid antigens are found in the epidermis and dermis. The aim of this study was to investigate the localisation of two major VZV glycoproteins (gpI and gpII) and of their respective genes in the skin. The distribution of VZV gpI and II in 27 formalin fixed paraffin embedded skin biopsies from herpes tester eruptions were compared by immunohistochemistry. Double immunostaining was carried our to identify infected cells. The presence of viral nucleic acids coding for gpI and gpII was examined by in situ hybridisation. The distribution of gpI and gpII and their corresponding genome sequences was similar in the epidermis, gpI and gpII were also detected in dermal FXIIIa positive dendrocytes, in Mac 387 and CD68 positive macrophages, and in perineural and endothelial cells. However, the corresponding viral nucleic acids were rarely and barely detected in these cells of the dermis. It is concluded that VZV infection of epithelial cells follows a different course than in dermal cells. (C) 1995 Wiley-Liss, Inc.
Disciplines :
Immunology & infectious disease Dermatology
Author, co-author :
Nikkels, Arjen ; Université de Liège - ULiège > Dermatologie
Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Debrus, S.
Sadzot-Delvaux, Catherine ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie et immunologie - GIGA-M : Coordination scientifique
Piette, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research
Rentier, Bernard ; Université de Liège - ULiège > Services administratifs généraux > Recteur - GIGA-R : Virologie - Immunologie
Pierard, Gérald ; Université de Liège - ULiège > Dermatopathologie
Language :
English
Title :
Distribution of varicella-zoster virus gpI and gpII and corresponding genome sequences in the skin
Arvin AM, Kinney‐Thomas E, Shriver K, Grose C, Koropchak CM, Scranton E, Wittek AE, Diaz PS (1986) Immunity to varicellazoster viral glycoproteins gpI (gp 90/58) and gpIII (gp 118) and to a nonglycosylated protein, p 170. Journal of Immunology 137:1346-1351.
Croen KD (1988) Latency and the consequence of reactivation of the varicella‐zoster virus. Annals of Internal Medicine 108:221-237.
Croen KD, Straus SE (1991) Varicella‐zoster virus latency. Annual Review of Microbiology 45:265-282.
Croen KD, Ostrove JM, Dragovic LJ, Straus SE (1988) Patterns of gene expression and sites of latency in human nerve ganglia are different for varicella‐zoster and herpes simplex viruses. Proceedings of the National Academy of Science 85:9773-9777.
Davison AJ, Scott JE (1986) The complete DNA sequence of varicella‐zoster virus. Journal of General Virology 67:1759-1816.
Davison AJ, Edson CM, Ellis RW, Forghani B, Gilden DH, Grose C, Keller PM, Vafai A, Wroblewska Z, Yamanishi K (1986) New common nomenclature for glycoprotein genes of varicella‐zoster virus and their glycosylated products. Journal of Virology 57:1195-1197.
Dubuisson J, Boulanger D, Bublot M, Thiry E, Pastoret PP (1989) Proteins specified by bovine herpes virus type 4: Structural proteins of the virion and identification of two major glycoproteins by using monoclonal antibodies. Journal of General Virology 70:1743-1753.
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclear fragments to high specific activity. Annals of Biochemistry 132:5-13.
Fleckenstein B, Müller I, Collins J (1982) Cloning of a complete human cytomegalovirus genome in cosmids. Gene 18:39-46.
Gilden DH, Vafai A, Shtram Y, Becker Y, Devlin M, Wellish M (1983) Varicella‐zoster virus DNA in human sensory ganglia. Nature 306:478-480.
Gilden DH, Rozenman Y, Murray R, Devlin M, Vafai A (1987) Detection of varicella‐zoster virus nucleic acid in neurons of normal human thoracic ganglia. Annals of Neurology 22:377-380.
Gilden DH, Devlin M, Wellish M, Mahalingham R, Huff C, Hayward A, Vafai A (1989) Persistence of varicella‐zoster DNA in blood mononuclear cells of patients with varicella or zoster. Virus Genes 2:299-305.
Grose C, Ng TI (1992) Intracellular synthesis of varicella‐zoster virus. Journal of Infectious Diseases 166:S7.
Hyman RW, Ecker JR, Tenser RB (1983) Varicella‐zoster virus RNA in human trigeminal ganglia. Lancet 2:814-816.
Keller PM, Lonergan K, Neff BJ, Morton DA, Ellis RW (1986) Purification of individual varicella‐zoster virus (VZV) glycoproteins gpI, gpII, and gpIII and their use in ELISA for detection of VZV glycoprotein‐specific antibodies. Journal of Virological Methods 14:177-188.
Litwin V, Sandor M, Grose C (1990) Cell surface expression of varicella‐zoster virus glycoproteins and Fc receptor. Virology 178:263-272.
Litwin V, Jackson W, Grose C (1992) Receptor properties of two varicella‐zoster glycoproteins gpI gpIV homologues to herpes simplex virus gE/gI. Journal of Virology 66:3643-3651.
Mahalingham R, Wellish M, Wolf W, Dueland AM, Cohrs R, Vafai A (1990) Latent varicella‐zoster viral DNA in human trigeminal and thoracic ganglia. New England Journal of Medicine 323:627-631.
Martin JR, Holt RK, Langston C, Gilden DH, Richardson EP, Manz HJ, Singer DB (1991) Type specific identification of herpes simplex and varicella‐zoster virus antigen in autopsy tissues. Human Pathology 22:75-80.
Meier JL, Straus SE (1992) Comparative biology of latent varicella‐zoster virus and herpes simplex virus infections. Journal of Infectious Diseases 166:13-23.
Merville‐Louis MP, Sadzot‐Delvaux C, Delree P, Piette J, Rentier B, Moonen G (1987) Molecular cloning of varicella‐zoster virus DNA and its detection in situ in infected nerve cells. Archives of International Physiology and Biochemistry 95:B87.
Muraki R, Baba T, Iwasaki T, Sata T, Kurata T (1992) Immunohistochemical study of skin lesions in herpes zoster. Virchows Archiv [A] 420:71-76.
Nikkels AF, Sadzot‐Delvaux C, Cloes JM, Rentier B, Piérard GE (1992) Granulomatous reactions following herpes zoster contain varicella‐zoster glycoprotein gpI. Journal of Investigative Dermatology 98:522.
Nikkels AF, Debrus S, Sadzot‐Delvaux C, Piette J, Delvenne P, Rentier B, Piérard GE (1993) Comparative immunohistochemical study of herpes simplex and varicella‐zoster infection. Virchows Archiv [A] 422:121-126.
Nuovo GJ, Richart RM (1989) A comparison of biotin‐ and 35S based in situ hybridization. Methodologies for detection of human papillomavirus DNA. Laboratory Investigation 61:471-476.
Schmitz GG, Walter T, Seibl R, Kessler C (1991) Non radioactive labeling of oligonucleotides in vitro with the hapten digoxyginin by tailing with terminal transferase. Annals of Biochemistry 192:222-231.
Snoeck R, Schols D, Sadzot‐Delvaux C, Cloes JM, Andrei G, Declerq E, Piette J, Rentier B (1992) Flow cytometric method for the detection of gpI antigens of varicella‐zoster virus and evaluation of anti‐VZV agents. Journal of Virological Methods 38:243-254.
Vafai A, Murray RS, Wellish M, Devlin M, Gilden DH (1988) Expression of varicella‐zoster virus and herpes simplex virus in normal human trigeminal ganglia. Proceedings of the National Academy of Science USA 85:2362-2366.
Vonsover A, Leventon‐Kriss S, Langer A, Smetana Z, Zaizov R, Potaznick D, Cohen J, Gotlieb‐Stematsky T (1987) Detection of varicella‐zoster virus in lymphocytes by DNA hybridization. Journal of Medical Virology , 57, 46; 21.
Weigle KA, Grose C (1983) Common expression of varicella‐zoster viral glycoprotein antigens in vitro and in chicken pox and zoster vesicles. Journal of Infectious Diseases 148:630-637.
Wigdahl B, Rong BL, Kinney‐Thomas E (1986) Varicella‐zoster virus infection of human sensory neurons. Virology 152:384-399.