Reference : Comparison of chitosan/siRNA and trimethylchitosan/siRNA complexes behaviour in vitro
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/41479
Comparison of chitosan/siRNA and trimethylchitosan/siRNA complexes behaviour in vitro
English
Dehousse, Vincent mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Garbacki, Nancy mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Jaspart, Séverine [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Castagne, Delphine mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Piel, Géraldine mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Colige, Alain mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Evrard, Brigitte mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
2010
International Journal of Biological Macromolecules
Butterworth Heinemann
46
342-349
Yes (verified by ORBi)
International
0141-8130
Guildford
United Kingdom
[en] Chitosan and trimethylchitosan (TMC)-siRNA nanoparticles were produced by simple complexation technique or by ionic gelation using tripolyphosphate (TPP). The obtained complexes were characterized in
terms of physicochemical properties such as size, zeta potential, complexation efficiency and stability. Furthermore, cytotoxicity, cell uptake and transfection efficiency of polyplexes were evaluated in
vitro. Under pH condition of cell culture medium, a strong decrease in siRNA condensation efficiency was observed with chitosan nanoparticles. This characteristic resulted in low transfection efficiencies in
HEK293 cell line. Formulation of chitosan polyplexes with TPP led to improvement of polyplexes stability but no significant increase in transfection efficiency was observed compared to simple chitosan
complexes. By contrast, TMC complexes did not have pH dependency on siRNA complexation. TMCsiRNA nanoparticles were stable in physiological condition. Accordingly, cellular uptake was increased
compared to chitosan polyplexes. However, improvement of transfection efficiency was low regarding to cellular uptake of these complexes. Chitosan and TMC complexes present some characteristics favourable for siRNA delivery, such as ability to integrate siRNA into small discrete particles or low toxicity of the complexes. This study also highlights the importance of complexes stability in physiological environment for siRNA transfection purposes.
http://hdl.handle.net/2268/41479

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