[en] The varicella-zoster virus (VZV) open reading frame 62 encodes an immediate-early protein (IE62) that transactivates expression of various VZV promoters and autoregulates its own expression in transient expression assays. In Vero cells, IE62 was shown to transactivate the expression of all putative immediate-early (IE) and early (E) genes of VZV with an up-regulating effect at low intracellular concentrations. To define the functional domains involved in the regulatory properties of IE62, a large number of in-frame insertions and deletions were introduced into a plasmid-borne copy of the gene encoding IE62. Studies of the regulatory activities of the resultant mutant polypeptides in transient expression assays allowed to delineate protein regions important for repression of its own promoter and for transactivation of a VZV putative immediate-early gene (ORF61) promoter and an early gene (ORF29) promoter. This mutational analysis resulted in the identification of a new functional domain situated at the border between regions 4 and 5 which plays a crucial role in the IE62 regulatory functions. This domain turned out to be very well conserved amongst homologous alphaherpesvirus regulatory proteins and appeared to be rich in bulky hydrophobic and proline residues, similar to the proline-rich region of the CAAT box binding protein CTF-1. By immunofluorescence, a nuclear localization signal has been mapped in region 3.
Disciplines :
Immunology & infectious disease
Author, co-author :
Baudoux, Laurence
Defechereux, Patricia
Schoonbroodt, Sonia
Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Rentier, Bernard ; Université de Liège - ULiège > Département de Microbiologie > Virologie fondamentale et Immunologie
Piette, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Language :
English
Title :
Mutational analysis of varicella-zoster virus major immediate-early protein IE62
Ostrove, J. M., (1990) Adv. Virus Res., 38, 45-98.
Davison, AJ., and Scot, U. E., (1986) J. Gen. Virol., 67, 1759-1816.
Honess, R. W., and Roizman, B., (1974) J. Virol., 14, 8-19.
Batterson, W., and Roizman, B., (1983) J. Virol., 46, 371-377.
Campbell, M. E. M., Palfreyman, J. W., and Preston, C. M., (1984) J. Mol. Biol., 180, 1-19.
Gerster, T., and. Rocder, R. G., (1988) Proc. Natl Acad. Sei. USA, 85, 6347-6351.
Machem, S., and Roizman, B., (1982) J. Virol, 44, 939-949.
O’Hare, P., and Goding, C. R., (1988) Cell, 52, 453-445.
Preston, C. M., Frame, JA. C., and Campbell, M. EM., (1988) Cell, 52, 425-434.
Honess, R. W., and Roizman, B., (1975) Proc. Natl Acad. Sei. USA, 72, 1276-1280.
Everett, R. D., (1987) Anticancer Res., 7, 589-604.
Shiraki, K., and Hyman, R. W., (1987) Virology, 156, 423-426.
Felser, J. M., Kinchington, P. R., Inshauspc, G., Straus, S. E., and Ostrove, J. M., (1988) J. Virol., 62, 2076-2082.
Forghani, B., Mahalingam, JL, Vafai, A., Hurst, J. W., and Dupuis, K. W., (1990) Virus Res., 16, 195-210.
Kinchington, P. R., Hougland, J. K., Arvin, A. M., Ruyechan, W. T., and Hay, J., (1992) J. Virol, 66, 359-366.
Cabirac, G. F., Mahalingam, R., Wellish, M., and Gilden, D. H., (1990) Virus Res., 15, 57-68.
Everett, R. D., (1984) EM BO J., 3, 3135-3141.
Inchauspe, G., Nagpal, S., and Ostrove, J. M., (1989) Virology, 173, 700-709.
Inshauspc, G., and Ostrove, J. M., (1989) Virology, 173, 710-714.
Peiera, J. R, Mosca, J. D., Ruyechan, W. T., and Hay, J., (1992) J. Virol., 66, 5298-5304.
Disney, G. H., McKee, T. A., Preston, CM., and Everett, R. D., (1990) J. Gen. Virol, 71, 2999-3003.
Perera, J. R, Mosca, R. D., Sadeghi-Zadeh, M., Ruyechan, W. T., and Hay, J., (1992) Virology, 191, 346-354.
Me Geoch, DJ., Dolam, A., Donalc, LS and Braver, D. H. K., (1986) Nucleic Acids Res., 14, 1727-1745.
Felser, J. M., Straus, S. E., and Ostrove, J. M., (1987) J. Virol, 61, 225-228.
Disney, G. H., and. Everett, R. D., (1990) J. Gen. Virol, 71, 897-906.
Longnecker, R., and Roizman, B., (1986) 7. Virol, 58, 583-591.
Preston, CM., (1979) 7. Virol, 29, 275-284.
Dixon, JLAF., and Schaffer, PA., (1980) J. Virol, 36, 189-203.
Smith, C. A., Bates, J., Rivera-Gonzales, JL., Gu, JL., and Deluca, J., (1993) J. Virol, 67, 4676-4687.
Cheung, A. K., (1989) Nucleic Acids. Res., 17, 4637-4646.
Vleek, C., Paèes, V. A., and Schwyzer, M (1989) Virus Genes, 2, 335-346.
Grundy, FJ., Bauman, R. P., and O’Callaghan, DJ., (1989) Virology, 172, 223-236.
Deluca, J.J. A., and Schaffer, P. A., (1988) J. Virol, 62, 732-743.
Deluca, J. A., and Schaffer, P. A., (1988) Nucleic Acids. Res., 15, 4491-4511.
Paterson, T., and Everett, R. D., (1990)7. Gen. Virol, 71, 1775-1783.
Paterson, T., and Everett, JCD., (1988) Virology, 166, 186-196.
Paterson, T., and Everctt, R. D., (1988) Nucleic Acids Res., 16, 11005-11025.
Shepard, A. A., Imbalzanop, A. M., and Deluca, N. A., (1989) J. Virol., 63, 3714-3728.
Faber, S. W., and Wilcox, K. W., (1986) Nucleic Acids Res., 14, 6067-6083.
Wu, C. L., and Wilcox, K. W., (1990) Nucleic Acids. Res., 18, 531-538.
Wu, C. L., and Wilcox, K. W., (1991) 7 Virol, 65, 1149-1159.
Resnick J., Boyd, B. A., and Haffey, M. L., (1989) J. Virol., 63, 2497-2503.
Roberts, M. S., Boundy, A., O’Hare, J’., Pizzomo, M-C., Ciufo, D. M., and Hayward, G. S., (1988) 7. Virol, 62, 4307-1320.
Beard, J’., Faber, S., Wilcox, K. W., and Pizer, L. I., (1986) Proc. Natl Acad. Sei. USA, 83, 4016-4020.
Tedder, D. G., Everett, R. D„ Wilcox, K. W., Beard, J., and Pizer, X. I., (1989) 7. Virol, 63, 2510-2520.
Mullen, M A, Ciufo, D. M., and Hayward, G. S., (1994) 7. Virol., 68, 3250-3266.
Tyler, J. K., and Everett, R. D., (1993) Nucleic Acids Res., 21, 513-522.
Cohen, J. I., Heffel, JJ., and Seidel, K., (1993)7. Virol., 67, 4246-4251.
Perera, J-P., Mosca, J. D., Ruyechan, W. T., Hayward, G. S., Straus, S. E., and Hay, J., (1993) 7. Virol, 67, 4474-4483.
Defechereux, R, Melen, L., Baudoux, L., Merville-Louis, M P. Rentierand Piette, J., (1993) J. Virol, 67, 4379-4385.
McKee, T. A., Disney, G. H., Everett, J. D., and Preston, C. M., (1990) J. Gen Virol, 71, 897-906.
Sambrook, J., Fntsch, E. F., and Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Neuman, J. R., Morency, C. A., and Russian, K. O., (1987) BioTechniques, 5, 444.
Kalderon, D., Roberts, R, Richardson, W. D., and Smith, A. E., (1984) Nature, 311, 33-38.
Moriuchi, JL, Moriuchi, M, Straus, S, and Cohen, J. I., (1993) J. Virol., 67, 2739-2746.
Monuchi, H., Moriuchi, M, Straus, S. E., and Cohen, J. I., (1994) Virology, 200, 297-300.
Pellem, P. E., McKnight, J. L. C., Jenkins, F. J., and Roizman, B., (1985) Proc. Natl. Acad Sei. USA, 82, 5870-5874.
Ling, R, Kinchingtom, P. R., Sadeghi-Zadeh, M, Ruyechan, W. T., and Hay, J., (1992) 7. Virol, 66, 3690-3698.
Tyler, J. K., Allen, JCE., and Everett, RD., (1994) Nucleic Acids Res., 22, 270-278.
Mermod, JI., O'Neill, E. A., Kelly, TJ., and Tjian, R., (1989) CeU, 58, 741-753.
Cress, W. D., and Triezenbeig, SJ., (1991) Science, 251, 87-90.
Kim, T. K., and Roeder, JU., (1994) Proc. Natl. Acad Sei. USA, 91, 4170-4174.