Association of two single-isomer anionic CD in NACE for the chiral and achiral separation of fenbendazole, its sulphoxide and sulphone metabolites: Application to their determination after in vitro metabolism

Rousseau, Anne; Gillotin, Florian; Chiap, Patrice; Crommen, Jacques; Fillet, Marianne; Servais, Anne-Catherine

doi:10.1002/elps.200900723

Article (Scientific journals)

Association of two single-isomer anionic CD in NACE for the chiral and achiral separation of fenbendazole, its sulphoxide and sulphone metabolites: Application to their determination after in vitro metabolism

Rousseau, Anne; Gillotin, Florian; Chiap, Patrice et al.

2010 • In Electrophoresis, 31, p. 1482-1487

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/41408

DOI
10.1002/elps.200900723

PubMed
20376816

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Keywords :

Benzimidazole compounds; In vitro metabolites; Nonaqueous capillary electrophoresis; Single-isomer anionic cyclodextrins

Abstract :

[en] A NACE method was developed for the separation of fenbendazole (FBZ), a prochiral drug giving rise to chiral (oxfendazole or OFZ) and nonchiral (FBZ sulphone or FBZSO2) metabolites. First, the effect of the nature and the concentration of CD as well as that of the acidic BGE on the enantiomeric separation of OFZ were studied. OFZ enantiomers were completely resolved using a BGE made up of 10 mM ammonium formate and 0.5 M TFA in methanol containing 10 mM heptakis(2,3-di-O-acetyl-6-O-sulfo)-beta-CD and 10 mM heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-CD. Moreover, the NACE method was found to be particularly well suited to the simultaneous determination of FBZ, OFZ enantiomers, and FBZSO2. Thiabendazole was selected as an internal standard. The CD-NACE potential was then evaluated for in vitro metabolism studies using FBZ as a model case. The OFZ enantiomers and FBZSO2 could be detected after incubation of FBZ in the phenobarbital-induced male rat liver microsomes systems.

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Rousseau, Anne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Gillotin, Florian; Université de Liège - ULiège > Pharmacologie clinique

Chiap, Patrice ; Université de Liège - ULiège > Pharmacologie clinique

Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Servais, Anne-Catherine ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Language :

English

Title :

Publication date :

2010

Journal title :

Electrophoresis

ISSN :

0173-0835

eISSN :

1522-2683

Publisher :

Vch Publishers, Weinheim, Germany

Volume :

Pages :

1482-1487

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Fonds Léon Fredericq [BE]

Available on ORBi :

since 07 April 2010

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