ORBi: Rousseau Anne - Association of two single-isomer anionic CD in NACE for the chiral and achiral separation of fenbendazole, its sulphoxide and sulphone metabolites: Application to their determination after in vitro metabolism

Reference : Association of two single-isomer anionic CD in NACE for the chiral and achiral separatio...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Pharmacy, pharmacology & toxicology
	To cite this reference:	http://hdl.handle.net/2268/41408

Title :	Association of two single-isomer anionic CD in NACE for the chiral and achiral separation of fenbendazole, its sulphoxide and sulphone metabolites: Application to their determination after in vitro metabolism
Language :	English
Author, co-author :	Rousseau, Anne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Gillotin, Florian [Université de Liège - ULG > > Pharmacologie clinique > >]
	Chiap, Patrice [Université de Liège - ULg > > Pharmacologie clinique >]
	Crommen, Jacques [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Fillet, Marianne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Servais, Anne-Catherine [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Publication date :	2010
Journal title :	Electrophoresis
Publisher :	Vch Publishers
Volume :	31
Pages :	1482-1487
Audience :	International
ISSN :	0173-0835
City :	Weinheim
Country :	Germany
Keywords :	[en] Benzimidazole compounds ; In vitro metabolites ; Nonaqueous capillary electrophoresis ; Single-isomer anionic cyclodextrins
Abstract :	[en] A NACE method was developed for the separation of fenbendazole (FBZ), a prochiral drug giving rise to chiral (oxfendazole or OFZ) and nonchiral (FBZ sulphone or FBZSO2) metabolites. First, the effect of the nature and the concentration of CD as well as that of the acidic BGE on the enantiomeric separation of OFZ were studied. OFZ enantiomers were completely resolved using a BGE made up of 10 mM ammonium formate and 0.5 M TFA in methanol containing 10 mM heptakis(2,3-di-O-acetyl-6-O-sulfo)-beta-CD and 10 mM heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-CD. Moreover, the NACE method was found to be particularly well suited to the simultaneous determination of FBZ, OFZ enantiomers, and FBZSO2. Thiabendazole was selected as an internal standard. The CD-NACE potential was then evaluated for in vitro metabolism studies using FBZ as a model case. The OFZ enantiomers and FBZSO2 could be detected after incubation of FBZ in the phenobarbital-induced male rat liver microsomes systems.
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
Permalink :	http://hdl.handle.net/2268/41408

File(s) associated to this reference

Fulltext file(s):

	File	Commentary	Version	Size	Access
Restricted access	MS.pdf		Publisher postprint	171.04 kB	Request copy

All documents in ORBi are protected by a user license.

Dspace v1.5