Reference : Association of two single-isomer anionic CD in NACE for the chiral and achiral separa...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/41408
Association of two single-isomer anionic CD in NACE for the chiral and achiral separation of fenbendazole, its sulphoxide and sulphone metabolites: Application to their determination after in vitro metabolism
English
Rousseau, Anne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Gillotin, Florian [Université de Liège - ULG > > Pharmacologie clinique > >]
Chiap, Patrice [Université de Liège - ULg > > Pharmacologie clinique >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
2010
Electrophoresis
Vch Publishers
31
1482-1487
Yes (verified by ORBi)
International
0173-0835
Weinheim
Germany
[en] Benzimidazole compounds ; In vitro metabolites ; Nonaqueous capillary electrophoresis ; Single-isomer anionic cyclodextrins
[en] A NACE method was developed for the separation of fenbendazole (FBZ), a prochiral
drug giving rise to chiral (oxfendazole or OFZ) and nonchiral (FBZ sulphone or FBZSO2)
metabolites. First, the effect of the nature and the concentration of CD as well as that of
the acidic BGE on the enantiomeric separation of OFZ were studied. OFZ enantiomers
were completely resolved using a BGE made up of 10 mM ammonium formate and
0.5 M TFA in methanol containing 10 mM heptakis(2,3-di-O-acetyl-6-O-sulfo)-beta-CD and
10 mM heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-CD. Moreover, the NACE method was
found to be particularly well suited to the simultaneous determination of FBZ, OFZ
enantiomers, and FBZSO2. Thiabendazole was selected as an internal standard. The
CD-NACE potential was then evaluated for in vitro metabolism studies using FBZ as a
model case. The OFZ enantiomers and FBZSO2 could be detected after incubation of
FBZ in the phenobarbital-induced male rat liver microsomes systems.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
http://hdl.handle.net/2268/41408

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