ORBi: Do Thi Thao - Precision study on capillary electrophoresis methods for metacycline

Reference : Precision study on capillary electrophoresis methods for metacycline


	Document type :	Scientific journals : Article
	Discipline(s) :	Physical, chemical, mathematical & earth Sciences : Chemistry Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/41034

Title :	Precision study on capillary electrophoresis methods for metacycline
Language :	English
Author, co-author :	Do Thi, Thao [> > > >]
	Pomponi, Romeo [> > > >]
	Gotti, Roberto [> > > >]
	Saevels, Jan [> > > >]
	Van Hove, Ben [> > > >]
	Van Ael, Willy [> > > >]
	Matthijs, Nele [> > > >]
	Vander Heyden, Yvan [> > > >]
	Djan'geing'a, Roland Marini [> > > >]
	Chiap, Patrice [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Hubert, Philippe [Université de Liège - ULg > > Chimie analytique >]
	Crommen, Jacques [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Fabre, Huguette [> > > >]
	Dehouck, Pieter [> > > >]
	Hoogmartens, Jos [> > > >]
	Van Schepdael, Ann [> > > >]
Publication date :	Jun-2006
Journal title :	Electrophoresis
Publisher :	Wiley
Volume :	27
Issue/season :	12
Pages :	2317-2329
Audience :	International
ISSN :	0173-0835
City :	Weinheim
Keywords :	[en] capillary electrophoresis ; inter-laboratory study ; intermediate precision study ; metacycline ; precision
Abstract :	[en] A CE method for metacycline (MTC) determination was investigated in an inter-laboratory experiment. Many problems were encountered in this study, most of which were related to the transfer of the method to different CE equipment. The reported problems could be classified into different categories: problems related to the precision, to the parameters in the protocol, and to the MTC peak shape. As the peak shape problem was partially responsible for the poor precision, a new CE method was developed in order to obtain a good MTC peak shape on all equipment. The precision of this new method for MTC determination was examined in an intermediate precision study, where the influence of the factors "time" and "equipment" was investigated. Although the new method could be transferred to different instruments, the precision remained poor mainly due to the contributions of the between-replicate and the between-injection variances.
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/41034

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