ORBi: Begon Dominique - Yin Yang 1 cooperates with activator protein 2 to stimulate ERBB2 gene expression in mammary cancer cells.

Reference : Yin Yang 1 cooperates with activator protein 2 to stimulate ERBB2 gene expression in mam...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Oncology Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/4088

Title :	Yin Yang 1 cooperates with activator protein 2 to stimulate ERBB2 gene expression in mammary cancer cells.
Language :	English
Alternative title :	[fr] YY1 coopère avec AP-2 pour stimuler l'expression du gène ERBB2 dans les cellules de cancer du sein
Author, co-author :	Begon, Dominique [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques - Laboratoire d'Oncologie Moléculaire > >]
	Delacroix, Laurence [Université de Liège - ULg > Département des sciences cliniques > Anatomie et cytologie pathologiques - Laboratoire d'Oncologie Moléculaire > >]
	Vernimmen, Douglas [University of Oxford > Institute of Molecular Medecine, John Radcliffe Hospital > Medical Research Council Molecular Haematology Unit > >]
	Jackers, Pascale [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques - Laboratoire d'Oncologie Moléculaire > >]
	Winkler, Rose [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques - Laboratoire d'Oncologie Moléculaire > >]
Publication date :	1-Jul-2005
Journal title :	Journal of Biological Chemistry
Publisher :	American Society for Biochemistry and Molecular Biology
Volume :	280
Issue/season :	26
Pages :	24428-34
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0021-9258
e-ISSN :	1083-351X
City :	Baltimore
Country :	MD
Keywords :	[en] Binding Sites ; Blotting, Western ; Breast Neoplasms/metabolism ; Cell Line ; Cell Line, Tumor ; Cell Nucleus/metabolism ; Chromatin/chemistry ; Chromatin Immunoprecipitation ; DNA-Binding Proteins/metabolism/physiology ; Erythroid-Specific DNA-Binding Factors ; Gene Expression Regulation, Neoplastic ; Genetic Vectors ; Humans ; Immunoprecipitation ; Luciferases/metabolism ; Models, Genetic ; Oligonucleotides, Antisense/chemistry ; Plasmids/metabolism ; Promoter Regions, Genetic ; Protein Binding ; RNA, Messenger/metabolism ; Receptor, erbB-2/biosynthesis ; Transcription Factor AP-2 ; Transcription Factors/metabolism/physiology ; Transcription, Genetic ; Transcriptional Activation ; Transfection ; YY1 Transcription Factor
Abstract :	[en] Overexpression of the ERBB2 oncogene is observed in about 30% of breast cancers and is generally correlated with a poor prognosis. Previous results from our and other laboratories indicated that elevated transcriptional activity contributes significantly to the overexpression of ERBB2 mRNA in mammary adenocarcinoma cell lines. Activator protein 2 (AP-2) transcription factors account for this overexpression through two recognition sequences located 215 and 500 bp upstream from the transcription start site. Furthermore, AP-2 transcription factors are highly expressed in cancer cell lines overexpressing ERBB2. In this report, we examined the cooperative effect of Yin Yang 1 (YY1) on AP-2-induced activation of ERBB2 promoter activity. We detected high levels of YY1 transcription factor in mammary cancer cell lines. Notably, we showed that YY1 enhances AP-2alpha transcriptional activation of the ERBB2 promoter through an AP-2 site both in HepG2 and in HCT116 cells, whereas a carboxyl-terminal-truncated form of YY1 cannot. Moreover, we demonstrated the interaction between endogenous AP-2 and YY1 factors in the BT-474 mammary adenocarcinoma cell line. In addition, inhibition of endogenous YY1 protein by an antisense decreased the transcription of an AP-2-responsive ERBB2 reporter plasmid in BT-474 breast cancer cells. Finally, we detected in vivo AP-2 and YY1 occupancy of the ERBB2 proximal promoter in chromatin immunoprecipitation assays. Our data thus provide evidence that YY1 cooperates with AP-2 to stimulate ERBB2 promoter activity through the AP-2 binding sites.
Research units :	Giga-Cancer
Funders :	Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq (Liège, Belgique) ; Télévie (Belgique) ; Centre Anticancéreux attaché à l'Université de Liège ; Fédération Belge contre le Cancer
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/4088
DOI :	10.1074/jbc.M503790200
Other URL :	http://www.jbc.org/cgi/content/full/280/26/24428

File(s) associated to this reference

Fulltext file(s):

	File	Commentary	Version	Size	Access
Open access	BegonDY_2005_postprint_auteur.pdf		Author postprint	441.72 kB	View/Open

Additional material(s):

	File	Commentary	Version	Size	Access
Restricted access	BegonDY_2005_suppdata.pdf	Supplementary Data	-	791.17 kB	Request copy

All documents in ORBi are protected by a user license.

Dspace v1.5