Purification Of Antifungal Lipopeptides By Reversed-Phase High-Performance Liquid-Chromatography

[en] A rapid procedure for the purification of antifungal lipopeptides from Bacillus subtilis, a potential agent for biocontrol of plant diseases, was tested. It consists of a solid-phase extraction on C18 gel followed by reversed-phase chromatography using a biocompatible PepRPC HR 515 column with a pharmacia fast protein liquid chromatographic system. This is a very effective method for isolating and fractionating iturin A and surfactin, two lipopeptides of different nature, co-produced by Bacillus subtilis strain S499. The presence of homologous lipopeptides was easily detected.

Disciplines :

Agriculture & agronomy

Author, co-author :

Razafindralambo, Hary ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Paquot, Michel ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Hbid, Choukri

Jacques, Philippe ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries

Destain, Jacqueline ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Thonart, Philippe ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles

Language :

English

Title :

Purification Of Antifungal Lipopeptides By Reversed-Phase High-Performance Liquid-Chromatography

Publication date :

1993

Journal title :

Journal of Chromatography. A

ISSN :

0021-9673

eISSN :

1873-3778

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

639

Issue :

Pages :

81-85

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 13 January 2011

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Bibliography

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