Reference : Purification Of Antifungal Lipopeptides By Reversed-Phase High-Performance Liquid-Chr...
Scientific journals : Article
Life sciences : Agriculture & agronomy
http://hdl.handle.net/2268/40791
Purification Of Antifungal Lipopeptides By Reversed-Phase High-Performance Liquid-Chromatography
English
Razafindralambo, Hary [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Paquot, Michel mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Hbid, Choukri [> > > >]
Jacques, Philippe [> > > >]
Destain, Jacqueline mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Thonart, Philippe mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
1993
Journal of Chromatography. A
Elsevier Science
639
1
81-85
Yes (verified by ORBi)
International
0021-9673
Amsterdam
The Netherlands
[en] A rapid procedure for the purification of antifungal lipopeptides from Bacillus subtilis, a potential agent for biocontrol of plant diseases, was tested. It consists of a solid-phase extraction on C18 gel followed by reversed-phase chromatography using a biocompatible PepRPC HR 515 column with a pharmacia fast protein liquid chromatographic system. This is a very effective method for isolating and fractionating iturin A and surfactin, two lipopeptides of different nature, co-produced by Bacillus subtilis strain S499. The presence of homologous lipopeptides was easily detected.
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/40791
also: http://hdl.handle.net/2268/61181
10.1016/0021-9673(93)83091-6

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