Reference : Localisation ultrastructurale d'hormones hypophysaires par reaction immunocytochimiqu...
Scientific journals : Article
Human health sciences : Endocrinology, metabolism & nutrition
Localisation ultrastructurale d'hormones hypophysaires par reaction immunocytochimique sur coupes fines d'adenomes.
[en] Ultrastructural localization of pituitary hormones by immunocytochemical reactions in ultrathin sections of adenomas
Beckers, Albert mailto [Université de Liège - ULg > Département des sciences cliniques > Endocrinologie >]
Courtoy, R. [ > > ]
Reznik, Michel [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Stevenaert, Achille [Centre Hospitalier Universitaire de Liège - CHU > > Neurochirurgie >]
Hennen, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >]
Boniver, Jacques [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Comptes Rendus des Séances de la Société de Biologie et de ses Filiales
Yes (verified by ORBi)
[en] Adenoma/ultrastructure ; Adrenocorticotropic Hormone/analysis ; Enzyme-Linked Immunosorbent Assay ; Growth Hormone/analysis ; Histological Techniques ; Humans ; Microscopy, Electron ; Pituitary Hormones/analysis ; Pituitary Neoplasms/ultrastructure ; Prolactin/analysis ; Radioimmunoassay
[en] Human pituitary adenomas were fixed in glutaraldehyde and embedded in epon. Ultrathin sections were incubated either with anti-hGH, anti-hPRL or anti-hLH. They were incubated with second step goat anti-rabbit immunoglobulins linked to gold particles. Two PRL secreting adenomas, one GH and PRL secreting adenoma, one ACTH secreting adenoma and two non secreting adenomas were studied. The specificity and the limits of the method were discussed in relation with the results obtained in light microscopy with the PAP method.
Researchers ; Professionals

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