[en] A sequential extraction of proteins from whole corn kernels dried between 54 and 130 degrees C was performed in order to elucidate the effect of the drying temperature on the solubility, the purity and the electrophoretic patterns of the different classes of corn proteins. It was observed that albumin, globulin and zein solubilities dropped significantly when the drying temperature increased, while fractions solubilised as glutelin-G(2) and glutelin-G3 increased until 110 degrees C before dropping slightly at 130 degrees C. The analysis of the solubility of different protein groups indicated that mechanisms other than the creation of new disulfide bonds between proteins occurred during the high temperature drying of corn. Except for glutelin-G1 and zein isolates, which were highly pure, the purities of albumin, globulin, glutelin-G2 and glutelin-G3 isolates after dialysis were influenced by the drying temperature. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed the disappearance of some water and salt-soluble poly-peptides at high drying temperatures. The electrophoretic patterns of zein and glutelin-G, were not significantly modified, although the solubility of zein was affected by the drying temperature. (c) 2008 Elsevier Ltd. All rights reserved.
Bewley, J.D., Black, M., (1978) Physiology and biochemistry of seeds in relation to germination, , Springer-Verlag, New York
Eckhoff, S.R., Wet Milling (2004) Encyclopedia of grain science, 2, pp. 225-241. , Wrigley C., and Walker C.E. (Eds), Elsevier Ltd, Amsterdam
Hall, C.W., (1980) Drying and storage of agricultural crops, , AVI, West port, CT
Hung, S.C., Zayas, J.F., Protein solubility, water retention, and fat binding of corn germ protein flour compared with milk proteins (1992) Journal of Food Science, 57 (2), pp. 372-376
Jayas, D.S., White, N.D.G., Storage and drying of grain in Canada: Low cost approaches (2003) Food Control, 14, pp. 255-261
Landry, J., La zéine du grain de maïs: Préparation et caractérisation (1979) Biochimie, 61, pp. 549-558
Landry, J., Delhaye, S., Di Goiai, L., Protein distribution in gluten products isolated during and after wet-milling of maize grains (1999) Cereal Chemistry, 76 (4), pp. 503-505
Landry, J., Moureaux, T., Hétérogénéité des Glutélines du grain de maïs: Extraction sélective et composition en acides aminés des trois fractions isolées (1970) Bulletin de la Societe de Chimie Biologique, 52 (10), pp. 1021-1037
Landry, J., Moureaux, T., Distribution and amino-acid composition of protein groups located in different histological parts of maize grain (1980) Journal of Agricultural and Food Chemistry, 28, pp. 1186-1191
Landry, J., Moureaux, T., Physicochemical properties of maize glutelins as influenced by their isolation conditions (1981) Journal of Agricultural and Food Chemistry, 29, pp. 1205-1212
Lasseran, J.C., Incidence of drying and storing conditions of corn (maize) on its quality for starch industry (1973) Starch, 25 (8), pp. 257-262
Li, J.S., Vasal, S.K., Quality protein maize (2004) Encyclopedia of grain science, 2, pp. 212-216. , Wrigley C., and Walker C.E. (Eds), Elsevier Ltd, Amsterdam
Lin, C.S., Zayas, J.F., Functionality of defated corn germ proteins in a model system: Fat binding capacity and water retention (1987) Journal of Food Science, 52 (5), pp. 1308-1311
Lupano, C.E., Añon, M.C., Denaturation of wheat proteins during drying (1986) Cereal Chemistry, 63 (3), pp. 259-262
McGuire, T.A., Earle, F.R., Changes in solubility of corn protein resulting from three artificial drying of high-moisture corn (1958) Cereal Chemistry, 35, pp. 179-188
Moueium, Z.A., El Tinay, A.H., Abdalla, A.W., Effect of germination on protein fraction of corn cultivars (1996) Food Chemistry, 57 (3), pp. 381-384
Neumann, P.E., Wall, J.S., Walker, C.E., Chemical and physical properties of proteins in wet-milled corn gluten (1984) Cereal Chemistry, 61 (4), pp. 353-356
Osborne, T.B., Mendel, L.B., Nutritive properties of proteins of the maize kernel (1914) The Journal of Biological Chemistry, 18 (1), pp. 1-16
Pallai, E., Németh, J., Mujumdar, A.S., Spouted bed drying (1987) Handbook of industrial drying, pp. 419-460. , Mujumdar A.S. (Ed), Marcel Dekker, Inc, New York
Paulis, J.W., Disulfide structures of zein proteins from corn endosperm (1981) Cereal Chemistry, 58 (6), pp. 542-546
Paulis, J.W., Recent developments in corn protein research (1982) Journal of Agricultural and Food Chemistry, 30, pp. 14-20
Paulis, J.W., James, C., Wall, J.S., Comparison of glutelin proteins in normal and high-lysine corn endosperms (1969) Journal of Agricultural and Food Chemistry, 17 (6), pp. 1301-1305
Paulis, J.W., Wall, J.S., Albumins and globulins in extracts of corn grain parts (1969) Cereal Chemistry, 46, pp. 263-273
Paulis, J.W., Wall, J., Comparison of the protein composition of selected corns and their wild relatives, teosinte and tripsacum (1977) Journal of Agricultural and Food Chemistry, 25 (2), pp. 265-270
Sodek, L., Wilson, C.M., Amino acid composition of proteins isolated from normal, opaque-2, and floury-2 corn endosperms by a modified Osborne procedure (1971) Journal of Agricultural and Food Chemistry, 19 (6), pp. 1144-1150
Townsend, A.-A., Nakai, S., Relationships between hydrophobicity and foaming characteristics of proteins (1983) Journal of Food Science, 48, pp. 588-594
Turner, J.E., Boundy, J.A., Dimler, R., Zein: A heterogeneous protein containing disulfide-linked aggregates (1965) Cereal Chemistry, 42, pp. 452-461
Wall, J.S., James, C., Donaldson, G.L., Corn proteins: Chemical and physical changes during drying of grain (1975) Cereal Chemistry, 52, pp. 779-790
Weller, C., Paulsen, M.R., Steinberg, M.P., Correlation of starch recovery with assorted quality factors of four corn hybrids (1988) Cereal Chemistry, 65 (5), pp. 392-397
Wight, A.W., Changes in properties of some maize cultivars associated with artificial drying at elevated temperatures. Part II. Protein solubility and other properties in relation to milling quality (1981) Starch, 33 (5), pp. 165-168