Reference : Systematic chromosomal aberrations found in murine bone marrow-derived mesenchymal st...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/40471
Systematic chromosomal aberrations found in murine bone marrow-derived mesenchymal stem cells.
English
Josse, Claire mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Génétique générale et humaine >]
Schoemans, R. [> > > >]
Niessen, Neville-Andrew mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau >]
Delgaudine, Marie mailto [Centre Hospitalier Universitaire de Liège - CHU > > Radiothérapie >]
Hellin, Anne-Cécile mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Herens, Christian mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Delvenne, Philippe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Bours, Vincent mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Aug-2010
Stem Cells & Development
Mary Ann Liebert, Inc.
19
8
1167-1173
Yes (verified by ORBi)
International
1547-3287
1557-8534
[en] Mesenchymal stem cells (MSCs) are studied as a cellular source for the treatment of various diseases. In this work, we isolated and cultivated murine bone marrow-derived MSCs. After a first observation of a solid tumour in a mouse injected with these cells, we systematically explored their chromosomal stability. We observed in all the cytogenetically analysed cases gross chromosomal alterations every time the MSCs went through the senescence crisis while the lymphocytes from the same animals showed a normal chromosome count. This observation was confirmed in different mouse strains, with different culture protocols, and even in short-term cultures after an hematopoietic cell negative immunodepletion performed in order to accelerate the isolation procedure. Therefore, we conclude that murine MSCs display high chromosomal instability, can generate tumours, and that care must be taken before using them for the evaluation of MSC therapeutic potential.
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/40471
10.1089/scd.2009.0264

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