Reference : Evaluation of a set of E. coli reporter strains as physiological tracer for estimatin...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/40277
Evaluation of a set of E. coli reporter strains as physiological tracer for estimating bioreactor hydrodynamic efficiency
English
Delvigne, Frank mailto [Université de Liège - ULg > > Gembloux Agro-Bio Tech >]
Ingels, Sophie [> >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles - Gembloux Agro-Bio Tech >]
2010
Process Biochemistry
Elsevier Science
45
11
1769-1778
Yes (verified by ORBi)
International
1359-5113
Oxford
United Kingdom
[en] scale-down ; fluorescent protein ; bioreactor
[en] A set of different green fluorescent protein (GFP) Escherichia coli reporter strains have been evaluated
in mini- and stirred bioreactors operating in fed-batch mode with different degrees of perturbations
in order to estimate their potential use as process-related stress biosensor. The mini-bioreactor platform
comprises a set of parallel shake flasks operating in fed-batch mode. The advantage of this system
is its high experimental throughput for the evaluation of the GFP synthesis capacity of our reporter
strains. In the case of classical shake flask system, no significant evolution of GFP synthesis have been
observed, considering the reduced microbial growth period allowed by the system, whereas in the case
of fed-batch operated mini-bioreactors, evolution of GFP synthesis, as well as GFP distribution among the
microbial population, has been observed for three preselected strains (prpoS, puspA and posmC::gfp).
More interestingly, a binary mode of expression has been observed in the case of the cultures carried
out with the reporter strains for which GFP synthesis is under the control of the rpoS promoter
which is induced under carbon limitation conditions. However, the generation of controlled glucose perturbations
is relatively limited in this system and, in a second step fully automated bioreactor with a
sclae-down strategy has been used to correlate the response of a prpoS::gfp strains with extracellular
glucose perturbations. In the case of the culture performed in perturbed bioreactor (glucose intermittent
feeding or glucose addition at the level of the recycle loop of a two-compartment scale-down bioreactor),
the slowdown of the GFP synthesis resulting in the observation of a binary repartition of GFP
content among the microbial population, has been observed. This observation led to the conclusion that
the prpoS::gfp can be used as a biosensor for the validation of a fed-batch profile in industrial-scale
bioreactors.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals
http://hdl.handle.net/2268/40277

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