[en] Cholecystokinin-like immunoreactivity has been demonstrated by radioimmunoassay and immunocytochemistry in the spinal cord of various mammals, in particular in nerve fibers of the superficial layers of the posterior column, but had not been detected in neuronal cell bodies. We report immunohistochemical evidence for the presence of a group of cholecystokinin-containing neuronal cell bodies in the lumbar spinal cord of the rat. This group of cells is only visualized after direct injection of colchicine into the spinal cord and is located near the central canal in the intermedio-medial nucleus of area X of Rexed.
Disciplines :
Neurosciences & behavior Neurology
Author, co-author :
Vanderhaeghen, J. J.
Deschepper, C.
Lotstra, F.
Vierendeels, G.
Schoenen, Jean ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Neuro-anatomie
Language :
English
Title :
Immunohistochemical evidence for cholecystokinin-like peptides in neuronal cell bodies of the rat spinal cord.
Publication date :
1982
Journal title :
Cell and Tissue Research
ISSN :
0302-766X
eISSN :
1432-0878
Publisher :
Springer Science & Business Media B.V., New York, United States - New York
Barber R.P., Vaughn J.E., Slemmon J.R., Salvaterra P.M., Roberts E., Leeman S.E. (1979) The origin, distribution and synaptic relationship of substance P axons in rat spinal cord. J Comp Neurol 184:331-352.
Beinfeld M.C. (1981) An HPLC and RIA analysis of the cholecystokinin peptide in rat brain. Neuropeptides 1:203-209.
Buffa R., Crivelli O., Fiocca R., Fontana P., Solcia E. (1979) Complement-mediated unspecific binding of immunoglobulins to some endocrine cells. Histochemistry 63:15-21.
Buijs R.M., Swaab D.F., Dogterom J., Van Leeuwen F.W. (1978) Intra- and extrahypothalamic vasopressin and oxytocin pathways in the rat. Cell Tissue Res 186:423-433.
Dockray G.J. (1976) Immunochemical evidence of cholecystokinin-like peptides in brain. Nature 264:568-570.
Go V.L.W., Yaksh T.L. (1980) Vasoactive intestinal peptide (VIP) and cholecystokinin CCK-8 in cat spinal cord and dorsal root ganglion: release from cord by peripheral nerve stimulation. Regulatory Peptides Suppl 1 2:43.
Grube D. (1980) Immunoreactivities of gastrin (G-) cells. II. Non specific binding of immunoglobulins to G-cells by ionic interactions. Histochemistry 66:149-167.
Grube D., Weber (1980) Immunoreactivities of gastrin (G-) cells. I. Dilution — dependent staining of G-cells by antisera and non-immune sera. Histochemistry 65:223-237.
Hökfelt T., Elde R., Johansson O., Terenius L., Stein L. (1977) The distribution of enkephalin-immunoreactive cell bodies in the rat central nervous system. Neurosci Lett 5:25-31.
Hökfelt T., Rbehfeld J.F., Skirboll L., Ivemark B., Goldstein M., Markey K. (1980) Evidence for coexistence of dopamine and CCK in meso-limbic neurones. Nature 285:476-478.
Hökfelt T., Skirboll L., Rehfeld J.F., Goldstein M., Markey K., Dann O. (1980) A subpopulation of mesencephalic dopamine neurones projecting to limbic areas contains a cholecystokinin-like peptide: evidence from immunohistochemistry combined with retrograde tracing. Neuroscience 5:2093-2124.
Innis R.B., Correa F.M.A., Uhl G.R., Schneider B., Snyder S.H. (1979) Cholecystokinin octapeptide-like immunoreactivity: histochemical localization in rat brain. Proc Natl Acad Sci USA 76:521-525.
Larsson L.I., Rehfeld J.F. (1979) Localization and molecular heterogeneity of cholecystokinin in the central and peripheral nervous system. Brain Res 165:201-218.
Lorén I., Alumets J., Håkanson R., Sundler F. (1979) Distribution of gastrin and CCK-like peptides in rat brain. Histochemistry 59:249-257.
Lundberg J.M., Hökfelt T., Nilsson G., Terenius L., Rehfeld J., Elde R., Said S. (1978) Peptide neurons in the vagus, splanchnic and sciatic nerves. Acta Physiol Scand 104:499-501.
.
Muller J.E., Strauss E., Yallow R.S. (1977) Cholecystokinin and its COOH-terminal octapeptide in the pig brain. Proc Natl Acad Sci (USA) 74:3035-3037.
Rehfeld J.F. (1978) Immunochemical studies on cholecystokinin. II. Distribution and molecular heterogeneity in the central nervous system and small intestin of man and hog. J Biol Chem 253:4016-4021.
Rexed B. (1964) Some aspects of the cytoarchitectonics and synaptology of the spinal cord. Organization of the spinal cord. Progr Brain Res Vol. 11 , J.C., Eccles, J.P., Schade, Elsevier, Amsterdam; 58-90.
Robberecht P., Deschodt-Lanckman M., Vanderhaeghen J.J. (1978) Demonstration of biological activity of brain gastrin-like peptide material in the human: its relationship with the COOH-terminal octapeptide of cholecystokinin. Proc Natl Acad Sci USA 75:524-528.
Schoenen J. L'organisation neuronale de la moelle épinière de l'homme , Sciences et Lettres, Pub., Liège; 1981, 356.
Sternberger L.A., Hardy P.H., Cuculis J.J., Meyer H.G. (1970) The unlabeled antibody-enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase — antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315-333.
Swanson L.W., Mc Kellar S. (1979) The distribution of oxytocin- and neurophysin-stained fibers in the spinal cord of the rat and monkey. J Comp Neurol 188:87-106.
Vanderhaeghen J.J., Lotstra F. (1979) Immunocytochemical localization of gastrin-cholecystokinin-like peptides in the brain and hypophysis of the rat. Neurosci Abstracts 5:543.
Vanderhaeghen J.J., Signeau J.J., Gepts W. (1975) New peptide in the vertebrate CNS reacting with antigastrin antibodies. Nature 257:604-605.
Vanderhaeghen J.J., De Mey J., Lotstra F., Gilles C. (1979) Localization of gastrin-cholecystokinin-like peptides in the brain and hypophysis of the rat. Symposium on Neural Peptides and Proteins, Brussels, December 9th, 1978. Acta Neurol Belg 79:62-63.
Vanderhaeghen J.J., Lotstra F., De Mey J., Gilles C. (1980) Immunohistochemical localization of cholecystokinin and gastrin-like peptide in the brain and hypophysis of the rat. Proceedings of the National Academy of Sciences 77:1190-1194.
Vanderhaeghen J.J. (1981) Gastrins and cholecystokinins in central nervous system and hypophysis. Hormones and cell regulation. European INSERM Symposium, Vol. 5 , J.E., Dumont, Numez, Elsevier/North Holland Biomedical Press, Amsterdam; 149-168.
.
Vandesande F., Dierickx K., De Mey J. (1977) The origin of the vasopressinergic and oxytocinergic fibres of the external region of the median eminence of the rat hypophysis. Cell Tissue Res 180:443-452.