Reference : Serine peptidase catalytic machinery: Cooperative one-step mechanism
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Mathematics
http://hdl.handle.net/2268/4007
Serine peptidase catalytic machinery: Cooperative one-step mechanism
English
Dive, Georges mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Dehareng, Dominique mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
1999
International Journal of Quantum Chemistry
John Wiley & Sons, Inc
73
2
161-174
Yes (verified by ORBi)
International
0020-7608
Hoboken
NJ
[en] acylation mechanism ; ab initio study ; beta-lactamases
[en] The acylation reaction of beta-lactamases by beta-lactam compounds is modeled as a one-step process. Twenty seven transition-state models are investigated at the restricted Hartree-Fock (RHF) level within the minimal MINI-1' basis set. These transition states differ by the nature of both the substrate and the amino acids constituting the reactive nucleophile. The intrinsic reactivity of the class-A and class-C beta-lactamases are under concern. Eight transition-state models were docked in a class-A beta-lactamase, TEM1, as optimized with the benzylpenicillin at the molecular mechanics level. In the proposed one-step acylation process, only two amino acids are directly involved, the usual nucleophilic serine, S70 in TEM1, and a close serine or tyrosine, S130 in TEM1, Y150 in P99. The lysine close to these two residues, K73 in TEM1, plays only an indirect role in the process. (C) 1999 John Wiley & Sons, Inc. Int J Quant Chem 73: 161-174, 1999.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Politique Scientifique Fédérale (Belgique) = Belgian Federal Science Policy
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/4007

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