Reference : An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/3983
An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira
English
[fr] Un protocole d'isolement d'ADN efficace pour les cyanobactéries filamenteuses du genre Arthrospira
Morin, Nicolas mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes >]
Vallaeys, Tatiana [ > > ]
Hendrickx, Larissa [ > > ]
Leys, Natalie [ > > ]
Wilmotte, Annick mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes >]
2010
Journal of Microbiological Methods
Elsevier Science
80
148-154
Yes (verified by ORBi)
International
0167-7012
Amsterdam
The Netherlands
[en] DNA isolation ; Arthrospira ; cyanobacteria
[en] Thanks to their photosynthetic and nutritive properties, cyanobacteria of the Arthrospira genus are of interest as food supplements, as efficient oxygen producing life support system organisms for manned space flight, and for the production of biofuels. Despite these potential valuable applications, full genome sequences and genetic information in general on Arthrospira remain scarce. This is mainly due to the difficulty to extract sufficient high molecular weight nucleic acids from these filamentous cyanobacteria.
In this article, an efficient and reproducible DNA extraction procedure for cyanobacteria of the genus Arthrospira was developed. The method is based on the combination of a soft mechanical lysis with enzymatic disruption of the cell wall. The comparison with other extraction protocols clearly indicates that this optimised method allows the recovery of a larger amount of DNA. Furthermore, the extracted DNA presents a high molecular weight, a reduced degradation and an excellent overall quality. It can be directly used for molecular biology purposes such as PCR, and clone library construction.
Centre d'Ingénierie des Protéines - CIP
StudieCentrum voor Kernenergie/Centre d'Etudes Nucléaires (SCK-CEN), Mol, Belgie ; European Space Agency (ESA)
Studies on the response to spaceflight related conditions in the cyanobacterium Arthrospira sp. PCC8005 using a genomic approach
Researchers
http://hdl.handle.net/2268/3983
10.1016/j.mimet.2009.11.012
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