Article (Scientific journals)
OPTICAL MULTICHANNEL ANALYSIS OF PROTOCHLOROPHYLLIDE PHOTOTRANSFORMATION IN DETERGENT-SOLUBILIZED ETIOPLAST MEMBRANES OF WHEAT
Franck, Fabrice; OUAZZANI, M. A.; DUJARDIN, E. et al.
1994In Zeitschrift für Naturforschung. C, Journal of Biosciences, 49 (1-2), p. 125-131
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Keywords :
ETIOPLAST; PROTOCHLOROPHYLLIDE; CHLOROPHYLLIDE; NADPH
Abstract :
[en] Extracts of wheat etioplast membranes obtained after treatment with 7 mM n-octyl-beta-D-glucopyranoside (OG), n-dodecyl-beta-D-maltoside (DM) or Triton X-100 contained the three spectral forms of Pchlide (the photoactive Pchlide(638) and Pchlide(638) and the inactive Pchlide(650)) in various relative amounds. The OG extract had a Pchlide composition close to that of the intact membranes whereas the DM extract was enriched in Pchlide(638) and the Triton extract was enriched in Pchlide(630). Measurements of the kinetics of phototransformation and of time-resolved absorbance spectra during phototransformation in continuous Light shows that the inactive Pchlide(630) is in fact slowly transformed to Chlide, especially in the Triton extract where this form is more abundant. Addition of NADPH favours the phototransformation of Pchlide(630) and the slow regeneration of Pchlide(638) and Pchlide(650) from Pchlide(630) in darkness after illumination. No such regeneration was however observed in the Triton extract. NADPH had only slight effects on the Chlide shift towards shorter wavelengths after phototransformation in solubilized membranes.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Franck, Fabrice ;  Université de Liège - ULiège > Biochimie végétale
OUAZZANI, M. A.;  Université de Liège - ULiège
DUJARDIN, E.;  Université de Liège - ULiège
POPOVIC, R.;  Université du Québec à Montréal
Language :
English
Title :
OPTICAL MULTICHANNEL ANALYSIS OF PROTOCHLOROPHYLLIDE PHOTOTRANSFORMATION IN DETERGENT-SOLUBILIZED ETIOPLAST MEMBRANES OF WHEAT
Publication date :
1994
Journal title :
Zeitschrift für Naturforschung. C, Journal of Biosciences
ISSN :
0939-5075
Publisher :
Verlag Z Naturforsch, Tubingen, Germany
Volume :
49
Issue :
1-2
Pages :
125-131
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 May 2010

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