Article (Scientific journals)
Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR.
Fischle, Wolfgang; Dequiedt, Franck; Hendzel, Michael J et al.
2002In Molecular Cell, 9 (1), p. 45-57
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Keywords :
Amino Acid Sequence; DNA Mutational Analysis; DNA-Binding Proteins/genetics/metabolism; Enzyme Activation; Hela Cells; Histone Deacetylases/genetics/metabolism; Humans; Molecular Sequence Data; Protein Binding; Repressor Proteins/genetics/metabolism; Transcription, Genetic
Abstract :
[en] Histone deacetylases (HDACs) play a key role in regulating eukaryotic gene expression. The HDAC domain, homologous to the yeast repressors RPD3 and HDA1, is considered necessary and sufficient for enzymatic activity. Here, we show that the catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor N-CoR/SMRT. All experimental conditions leading to the suppression of HDAC4 binding to SMRT/N-CoR and to HDAC3 result in the loss of enzymatic activity associated with HDAC4. In vitro reconstitution experiments indicate that HDAC4 and other class II HDACs are inactive in the context of the SMRT/N-CoR-HDAC3 complex and do not contribute to its enzymatic activity. These observations indicate that class II HDACs regulate transcription by bridging the enzymatically active SMRT/N-CoR-HDAC3 complex and select transcription factors independently of any intrinsic HDAC activity.
Disciplines :
Genetics & genetic processes
Biochemistry, biophysics & molecular biology
Author, co-author :
Fischle, Wolfgang 
Dequiedt, Franck   ;  Université de Liège - ULiège > GIGA-Research
Hendzel, Michael J
Guenther, Matthew G
Lazar, Mitchell A
Voelter, Wolfgang
Verdin, Eric
 These authors have contributed equally to this work.
Language :
English
Title :
Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR.
Publication date :
2002
Journal title :
Molecular Cell
ISSN :
1097-2765
eISSN :
1097-4164
Publisher :
Cell Press, Cambridge, United States - Massachusetts
Volume :
9
Issue :
1
Pages :
45-57
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 January 2010

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