[en] The effects of estradiol and two synthetic progestins (ORG2058 and R5020) on the expression of the high-affinity, metastasis-associated laminin receptor in two human breast carcinoma cell lines were examined. The T47D cell line contains estrogen and progesterone receptors, but the MDA-MB 231 cell line lacks both receptors. Treatment of T47D cells with 10(-9) M estradiol alone results in a three-fold increase (P less than or equal to .05) in the steady-state level of laminin receptor mRNA determined by RNA blot analysis as well as in cell-surface, laminin receptor expression that is evaluated by immunofluorescence. No effects of estradiol on the receptor-negative MDA-MB 231 cells were observed. Untreated and steroid-treated MDA-MB 231 cells had higher levels of laminin receptor mRNA than did untreated or estradiol-treated T47D cells. A more dramatic increase (five-fold; P less than or equal to .005) of mRNA and cell-surface expression in T47D cells was observed after treatment with estradiol plus 10(-8) M progestin or with progestin alone. Estradiol treatment also increased chemotaxis and haptotaxis of T47D cells but not of MDA-MB 231 cells to laminin; it had no effect on the attachment of these latter cells to laminin. Interestingly, treatment with estradiol plus progestin or progestin alone significantly increased the attachment of T47D cells to laminin but did not have an effect on either haptotaxis or chemotaxis to laminin. These results suggest that the various cell-laminin interactions are mediated by different mechanisms. The augmentation of laminin receptor mRNA by estrogen and progesterone treatment in hormone receptor-positive cells, but not in cells that lack these receptors, may relate functionally to the difference in the clinical aggressiveness between classes of breast cancers.
Castronovo, Vincenzo ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases
Taraboletti, G.
Liotta, L. A.
Sobel, M. E.
Language :
English
Title :
Modulation of laminin receptor expression by estrogen and progestins in human breast cancer cell lines.
Publication date :
1989
Journal title :
Journal of the National Cancer Institute
ISSN :
0027-8874
eISSN :
1460-2105
Publisher :
Oxford University Press, Cary, United States - North Carolina
PAST AN I, WILLINGHAM MC, FITZGERALD DJ: Immunotoxins. Cell 47:641-648, 1986
FRANKEL AE, HOUSTON LL, ISSELL BF: Prospects for immunotoxin therapy in cancer. Ann Rev Med 37:125-142, 1986
BLAKEY DC, THORPE PE: Prevention of carbohydrate-mediated clearance of ricin-containing immunotoxins by the liver. In Immunotoxins (Frankel AE, ed). Norwell, MA: Kluwer Academic Pub, 1988, pp 457-474
HARKONEN S, STOUDEMIRE J, MISCHAK R, ET AL: Toxicity and immunogenicity of monoclonal antimelanoma antibody-ricin A chain immunotoxin in rats. Cancer Res 47:1377-1382, 1987
LETVIN NL, CHALIFOUX LV, REIMANN KA, ET AL: In vivo administration of lymphocyte-specific monoclonal antibodies in nonhuman primates: Delivery of ribosome- inactivating proteins to spleen and lymph node T cells. J Clin Invest 78:666-673, 1986
BLAKEY DC, WATSON GJ, KNOWLES PP, ET AL: Effect of chemical deglycosylation of ricin A chain on the in vivo fate and cytotoxic activity of an immunotoxin composed of ricin A chain and anti-Thy 1.1 antibody. Cancer Res 47:947-952, 1987
SPITLER LE, DEL RIO M, KHENTIGAN A, ET AL: Therapy of patients with malignant melanoma using a monoclonal antimelanoma antibodyricin A chain immunotoxin. Cancer Res 47:1717-1723, 1987
HERTLER AA, SCHLOSSMAN DM, BOROWITZ MJ, ET AL: A phase I study of TlOl-ricin A chain immunotoxin in refractory chronic lymphocytic leukemia. J of Biol Response Mod 7:97-113, 1988
KERNAN NA, BYERS V, SCANNON PJ, ET AL: Treatment of steroidresistant acute graft-vs-host disease by in vivo administration of an anti-T-cell ricin A chain immunotoxin. JAMA 259:3154-3157, 1988
KOHLER G, MILSTEIN C: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495-497, 1975
BJORN MJ, RING D, FRANKEL AE: Evaluation of monoclonal antibodies for the development of breast cancer immunotoxins. Cancer Res 45:1214 1221, 1985
KING TP, LI Y, KOCHOUMIAN L: Preparation of protein conjugates via intermolecular disulfide bond formation. Biochemistry 17:1499-1505, 1978
FRAKER DJ, SPECK JC: Protein and cell membrane iodination with a sparingly soluble chloramide, l, 3, 4, 6-tetrachloro-3a, 6adiphenylglycoluril. Biochem Biophys Res Commun 80:849, 1978
Hsu SM, RAINE L, FANGER H: The use of avidin biotin peroxidase complex (ABC) in immunoperoxidase techniques. J Histochem Cytochem 29:577-580, 1981
HERTLER AA, SPITLER LE, FRANKEL AE: Humoral immune response to a ricin A chain immunotoxin in patients with metastatic melanoma. Cancer Drug Deliv 4:245-253, 1987
DICKSON RB, LIPPMAN ME: Estrogenic regulation of growth and polypeptide growth secretion in human breast carcinoma. Endocrinol Rev 8:29-43, 1987
BRAUNSBERG H, COLDHAM NG, WONG W: Hormonal therapies for breast cancer Can progesterone stimulate growth? Cancer Lett 30:213-218, 1986
BRAUNSBERG H, COLDHAM NG, LEAKE RE, ET AL: Actions of a progestogen on human breast cancer cells: Mechanisms of growth stimulation and inhibition. Eur J Cancer 23:563-571, 1986
HISSOM JR, MOORE MR: Progestin effects on growth in the human breast cancer cell line T47D: Possible therapeutic implication.Biochem Biophys Res Comraun 145:705-711, 1987
KLEDZIK GS, BRADLEY CJ, MEITES J: Reduction of carcinogen-induced mammary cancer incidence in rats by early treatment with hormones or drugs. Cancer Res 34:2953-2956, 1974
VIGNON F, BARDON S, CHABLOS D, ET AL: Antiestrogenic effect of R5020, a synthetic progestin in human breast cancer cells in culture. J Clin Endocrinol Metab 56:1124-1130, 1983
HORWITZ KB, FREIDENBERG GR: Growth inhibition and increase of insulin receptors in antiestrogen-resistant T47D human breast cancer cells by progestins: Implications for endocrine therapies. Cancer Res 45:167-173, 1985
SEDLACEK SM, HORWITZ KB: The role of progestins and progesterone receptors in the treatment of breast cancer. Steroids 44:467-484, 1984
POSTE G, FIDLER I: The pathogenesis of cancer metastases. Nature 283:139-146, 1980
LIOTTA LA, RAO CN, BARSKY SH: Tumor invasion and the extracellular matrix. Lab Invest 49:639-649, 1983
LIOTTA LA: Tumor invasion and metastases—role of the extracellular matrix: Rhoads Memorial Award Lecture. Cancer Res 46:1-7, 1986 TIMPL R, RHODE H, ROBEY PG, ET AL: Laminin: A glycoprotein from basement membranes. J Biol Chem 254:9933-9941, 1979
TERRANOVA VP, RAO CN, KABELIC T, ET AL: Laminin receptor on human breast carcinoma cells. Proc Natl Acad Sci USA 80:444-451, 1983
RAO CN, BARSKY SH, TERRANOVA VP, ET AL: Isolation of a tumor cell laminin receptor. Biochem Biophys Res Commun 111:804-808, 1983
MALINOFF H, WICHA MS: Isolation of a cell surface receptor protein for laminin from murine fibrosarcoma cells. J Cell Biol 96:1475-1480, 1983
HORAN HAND P, THOR A, SCHLOM J, ET AL: Expression of laminin receptor in normal and carcinomatous human tissues as defined by a monoclonal antibody. Cancer Res 45:2713-2719, 1985
WEVER UM, LIOTTA LA, JAYE M, ET AL: Altered levels of laminin receptor mRNA in various human carcinoma cells have different abilities to bind laminin. Proc Natl Acad Sci USA 83:7137-7141, 1986
Yow H, WONG JM, CHEN HS, ET AL: Increased mRNA expression of a laminin-binding protein in human colon carcinoma: Complete sequence of a full-length cDNA encoding the protein. Proc Natl Acad Sci USA 85:6394-6398, 1988
BARSKY SH, SIEGAL G, JANNOTTA F, ET AL: LOSS of basement membrane components by invasive tumors but not by their benign counterparts. Lab Invest 49:140-148, 1983
WEWER UM, TARABOLETTI G, SOBEL ME, ET AL: Role of laminin receptor in tumor cell migration. Cancer Res 47:5691-5698, 1987
ENGEL LW, YOUNG NA: Human breast carcinoma cells in continuous culture: A review. Cancer Res 38:4327-4336, 1978
DARBRE P, YATES J, CURTIS S, ET AL: Effect of estradiol on human breast cancer cells in culture. Cancer Res 43:349-354, 1983
WILLINGHAM MC, PASTAN IH: An Atlas of Immunofluorescence in Cultured Cells. New York: Academic Press, 1985
CASTRONOVO V, FOIDART JM, Li VECCHI M, ET AL: Human anti-agalactosyl IgG reduces the lung colonization by murine MO4 cells. Invasion Metastasis 7:325-345, 1987
SUGARMAN BJ, AGGARWALL BB, HASS PE, ET AL: Recombinant human tumor necrosis factor-a: Effects on proliferation of normal and transformed cells in vitro. Science 230:943-945, 1985
POSTLETHWAITE AE, SNYDERMAN R, KANG AH: The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor. J Exp Med 144:1188 1203, 1986
SOBEL ME, YAMAMOTO T, DE CROMBRUGGHE B, ET AL: Regulation of procollagen messenger ribonucleic acid levels in Rous sarcoma virustransformed chick embryo fibroblasts. Biochemstry 20:2678-2684, 1981
CLEVELAND DW, LOPATA MA, MACDONALD RJ, ET AL: Number and evolutionary conservation of a- and /5-tubulin and cytoplasmic /3- and y-actin genes using specific cloned cDNA probes. Cell 20:95-105, 1980
CHALBOS D, VIGNON F, KEYDAR I, ET AL: Estrogens stimulate cell proliferation and induce secretory proteins in a human breast cancer cell line (T47D). J Clin Endocrinol Metab 55:278-283, 1982
KLEINMAN HK, CANNON FB, LAURIE GW, ET AL: Biological activities of laminin. J Cell Biochem 27:317-325, 1985
ALBINI A, GRAF J, KITTEN GT, ET AL: 17-beta-Estradiol regulates and v-Ha-ras transfection constitutively enhances MCF7 breast cancer cell interactions with basement membranes. Proc Natl Acad Sci USA 83:8182-8186, 1986
BERTHOIS Y, KATZENELLENBOGEN JA, KATZENELLENBOGEN BS: Phenol red in tissue culture media is a weak estrogen: Implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA 83:2496-2500, 1986
CLEGG DO, HELDER JC, HANN BC, ET AL: Amino acid sequence and distribution of mRNA encoding a major skeletal muscle laminin binding protein: An extracellular matrix-associated protein with an unusual COOH-terminal polyaspartate domain. J Cell Biol 107:699-705, 1988
KLEINMAN HK, OGLE RC, CANNON FB, ET AL: Laminin receptors for neurite formation. Proc Natl Acad Sci USA 85:1282-1286, 1988
HORWITZ A, DUGGAN K, GREGGS R, ET AL: The cell substrate attachment (CSAT) antigen has properties of a receptor for laminin and fibronectin. J Cell Biol 101:2134-2144, 1985
GEHLSEN KR, DILLNER L, ENGVALL E, ET AL: The human laminin receptor is a member of the integrin family of cell adhesion receptors. Science 241:1228-1229, 1988
MURPHY LJ, SUTHERLAND RL, STEAD B, ET AL: Progestin regulation of epidermal growth factor receptor in human mammary carcinoma cells. Cancer Res 46:728-734, 1986
CHABLOS D, ROCHEFORT H: A 250 kilodalton cellular protein is induced by progestins in two human breast cancer cell lines MCF7 and T47D. Biochem Biophys Res Commun 121:421-427, 1984
MURPHY LC, MURPHY LJ, SHIU RPC: Progestin regulation of EGFmRNA accumulation in T47D human breast cancer cells. Biochem Biophys Res Commun 150:192-196, 1988
FISHER B, REDMOND C, FISHER EF, ET AL: Relative worth of estrogen or progesterone receptor and pathologic characteristics of differentiation as indicators of prognosis in node negative breast cancer patients: Findings from National Surgical Adjuvant Breast and Bowel Project Protocol B-06. J Clin Oncol 6:1076-1087, 1988
