[en] A new Bifidobacterium species is described based on the study of ten Gram-positive strains with fructose-6-phosphate phosphoketolase activity. They are part of a phenotypic group comprising 141 strains isolated from raw milk and raw milk cheeses in French raw milk cheese factories. This group was separated by a numerical analysis based on API 50CH, API 32A tests and growth at 46 degrees C. A strong similarity of 16S rRNA sequences (99.8%) was shown between strain FR62/b/3(T) and Bifidobacterium psychraerophilum LMG 21775(T). However, low DNA-DNA relatedness was observed between their DNAs (31%). The new isolates are able to grow at low temperatures (all ten strains up to 5 degrees C) and strain FR62/b/3(T) grows under aerobic conditions, as does B. psychraerophilum. However, contrary to B. psychraerophilum, they do not ferment L-arabinose, D-xylose, arbutin or melezitose, but they do acidify lactose. The DNA G+C content of FR62/b/3(T) is 56.4mol%. Therefore, the name Bifidobacterium crudilactis sp. nov. is proposed, with its type strain being FR62/b/3(T) (=LMG 23609(T)=CNCM I-3342(T)).
Disciplines :
Microbiology Food science
Author, co-author :
Delcenserie, Véronique ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaire
Gavini, Françoise; Institut National de la Recherche Agronomique > Laboratoire de Génie des Procédés et Technologie Alimentaires
Beerens, Henri; Centre d’Enseignement et de Recherche en Microbiologie Pharmaceutique, Lille
Tresse, O.; Institut National de la Recherche Agronomique > Laboratoire de Génie des Procédés
Franssen, Christine ; Université de Liège - ULiège > Sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Language :
English
Title :
Description of a new species, Bifidobacterium crudilactis sp. nov., isolated from raw milk and raw milk cheeses.
Altschul S.F., Madden T.L., Schäffer A.A., Zang J., Zang Z., Miller W., and Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search program. Nucleic Acids Res. 25 (1997) 3389-3402
Beerens H., Hass Brac de la Perriere B., and Gavini F. Evaluation of the hygienic quality of raw milk based on the presence of bifidobacteria: the cow as a source of faecal contamination. Int. J. Food Microbiol. 54 (2000) 163-169
Biavati B., Vescovo M., Torriani S., and Bottazi V. Bifidobacteria: history, ecology, physiology and applications. Ann. Microbiol. 50 (2000) 117-131
De Ley J., Cattoir H., and Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12 (1970) 133-142
Delcenserie V., Bechoux N., China B., Daube G., and Gavini F. A PCR method for detection of bifidobacteria in raw milk and raw milk cheese: comparison with culture-based methods. J. Microbiol. Methods 61 (2005) 55-67
Dong X., Xin Y., Jian W., Liu X., and Ling D. Bifidobacterium thermacidophilum sp. nov., isolated from an anaerobic digester. Int. J. Syst. Evol. Microbiol. 50 Pt 1 (2000) 119-125
Gavini F., and Beerens H. Origin and identification of bifidobacteria strains isolated from meat and meat products. Int. J. Food Microbiol. 46 (1999) 81-85
Gavini F., Delcenserie V., Kopeinig K., Pollinger S., Beerens H., Bonaparte C., and Upmann M. Bifidobacterium species isolated from animal feces and from beef and pork meat. J. Food Prot. 69 (2006) 871-877
Gevers D., Cohan F.M., Lawrence J.G., Spratt B.G., Coenye T., Feil E.J., Stackebrandt E., Van de Peer Y., Vandamme P., Thompson F.L., and Swings J. Opinion: re-evaluating prokaryotic species. Nat. Rev. Microbiol. 3 (2005) 733-739
Jian W., and Dong X. Transfer of Bifidobacterium inopinatum and Bifidobacterium denticolens to Scardovia inopinata gen. nov., comb. nov., and Parascardovia denticolens gen. nov., comb. nov., respectively. Int. J. Syst. Evol. Microbiol. 52 (2002) 809-812
Marmur J. A procedure for isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3 (1961) 208-218
Marmur J., and Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation. J. Mol. Biol. 5 (1962) 109-118
Masco L., Ventura M., Zink R., Huys G., and Swings J. Polyphasic taxonomic analysis of Bifidobacterium animalis and Bifidobacterium lactis reveals relatedness at the subspecies level: reclassification of Bifidobacterium animalis as Bifidobacterium animalis subsp. animalis subsp. nov. and Bifidobacterium lactis as Bifidobacterium animalis subsp. lactis subsp. nov. Int. J. Syst. Evol. Microbiol. 54 (2004) 1137-1143
Saitou N., and Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 (1987) 406-425
Sakata S., Kitahara M., Sakamoto M., Hayashi H., Fukuyama M., and Benno Y. Unification of Bifidobacterium infantis and Bifidobacterium suis as Bifidobacterium longum. Int. J. Syst. Evol. Microbiol. 52 (2002) 1945-1951
V. Scardovi. Irregular nonsporing gram-negative rods. Genus Bifidobacterium. Orla-Jensen 1924, in: P.H.A. Sneath, N.S. Mair, M.E. Sharpe, J.G. Holt (Eds.), Bergey's Manual of Systematic Bacteriology, Williams and Wilkins Co., Baltimore, 1986, pp. 1418-1434.
Simpson P.J., Ross R.P., Fitzgerald G.F., and Stanton C. Bifidobacterium psychraerophilum sp. nov. and Aeriscardovia aeriphila gen. nov., sp. nov., isolated from a porcine caecum. Int. J. Syst. Evol. Microbiol. 54 (2004) 401-406
Simpson P.J., Stanton C., Fitzgerald G.F., and Ross R.P. Genomic diversity and relatedness of bifidobacteria isolated from a porcine cecum. J. Bacteriol. 185 (2003) 2571-2581
Sneath P.H.A., and Sokal R.R. Numerical Taxonomy: The Principles and Practice of Numerical Classification (1973), W.H. Freeman, San Francisco
Stackebrandt E., Frederiksen W., Garrity G.M., Grimont P.A., Kampfer P., Maiden M.C., Nesme X., Rossello-Mora R., Swings J., Truper H.G., Vauterin L., Ward A.C., and Whitman W.B. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int. J. Syst. Evol. Microbiol. 52 (2002) 1043-1047
Stackebrandt E., and Goebel B.M. A Place for DNA-DNA reassociation and 16 S ribosomal-RNA sequence-analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44 (1994) 846-849
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., and Higgins D.G. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24 (1997) 4876-4882
Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore W.E.C., Murray R.G.E., Stackebrandt E., Starr M.P., and Trüper H.G. Report of ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37 (1987) 463-464
Yaeshima T., Fujisawa T., and Mitsuoka T. Bifidobacterium globosum, subjective synonym of Bifidobacterium pseudolongum and description of Bifidobacterium pseudolongum subsp. pseudolongum comb. nov. and Bifidobacterium pseudolongum subsp. globosum comb. nov. System. Appl. Microbiol. 15 (1992) 380-385
Zhu L., Li W., and Dong X. Species identification of genus Bifidobacterium based on partial HSP60 gene sequences and proposal of Bifidobacterium thermacidophilum subsp. porcinum subsp. nov. Int. J. Syst. Evol. Microbiol. 53 (2003) 1619-1623
