ORBi: Delcenserie Véronique - Bifidobacteria as indicators of faecal contamination along a sheep meat production chain

Reference : Bifidobacteria as indicators of faecal contamination along a sheep meat production chain


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Food science Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/2268/391

Title :	Bifidobacteria as indicators of faecal contamination along a sheep meat production chain
Language :	English
Author, co-author :	Delcenserie, Véronique [Université de Liège - ULg > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaire]
	Loncaric, D. [University of Veterinary Medicine Vienna > Meat Technology and Food Science > Institute of Meat Hygiene > >]
	Bonaparte, Christine [University of Veterinary Medicine Vienna, > Meat Technology and Food Science, > Institute for Meat Hygiene > >]
	Upmann, Mathias [University of Veterinary Medicine Vienna > Meat Technology and Food Science > Institute of Meat Hygiene > >]
	China, Bernard [Université de Liège - ULg - ULG > Sciences des Denrées alimentaires > Microbiologie des denrées alimentaires > >]
	Daube, Georges [Université de Liège - ULg > Sciences des Denrées alimentaires > Microbiologie des denrées alimentaires >]
	Gavini, Françoise [Institut Scientifique de Recherche Agronomique - INRA > Microbiologie et Chaîne Alimentaire, Villeneuve d'Ascq > > >]
Publication date :	2008
Journal title :	Journal of Applied Microbiology
Publisher :	Blackwell Publishing
Volume :	104
Issue/season :	1
Pages :	276-284
Audience :	International
ISSN :	1364-5072
e-ISSN :	1365-2672
City :	Oxford
Country :	United Kingdom
Keywords :	[en] Bifidobacterium choerinum ; Bifidobacterium pseudolongum ; Bifidobacterium thermophilum ; Chaperonin 60/genetics ; Colony Count, Microbial ; DNA-DNA hybridization ; Escherichia coli/isolation & purification ; faecal indicators ; hsp60 gene ; meat ; mupirocin ; PCR ; sheep ; slaughter
Abstract :	[en] Aims: The potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. The levels of bifidobacteria were compared with those of Escherichia coli. Total viable counts were followed along the chain (244 samples). Methods and Results: Forty-three per cent of the samples contained bifidobacteria, of which 15% were solely detected using a PCR method based on the hsp60 gene and not by a culture-based method. Bifidobacteria were detected in only three of nine sheep faeces samples using one or the other method. However, carcasses (types C and E) were highly contaminated. These sample types (30% and 28%, respectively) were positive for bifidobacteria and negative for E. coli. The species Bifidobacterium pseudolongum and Bif. thermophilum, isolated from faecal samples, were predominant. Bifidobacterium choerinum were found in C, D, E and F sample types. Conclusions: Bifidobacteria were shown more efficient than E. coli in carcasses samples. The presence of Bif. choerinum suggested a faecal pork contamination. Significance and Impact of the Study: Detection and identification of bifidobacteria, in correlation with E. coli counting, should improve hygiene quality of mutton processing chains.
Funders :	Commission européenne : Direction générale de la Recherche
Name of the research project :	BIFID
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/391
	also: http://hdl.handle.net/2268/111748
DOI :	10.1111/j.1365-2672.2007.03551.x

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