Reference : Bifidobacteria as indicators of faecal contamination along a sheep meat production chain
Scientific journals : Article
Life sciences : Food science
Life sciences : Microbiology
http://hdl.handle.net/2268/391
Bifidobacteria as indicators of faecal contamination along a sheep meat production chain
English
Delcenserie, Véronique mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaire]
Loncaric, D. [University of Veterinary Medicine Vienna > Meat Technology and Food Science > Institute of Meat Hygiene > >]
Bonaparte, Christine [University of Veterinary Medicine Vienna, > Meat Technology and Food Science, > Institute for Meat Hygiene > >]
Upmann, Mathias [University of Veterinary Medicine Vienna > Meat Technology and Food Science > Institute of Meat Hygiene > >]
China, Bernard [Université de Liège - ULg - ULG > Sciences des Denrées alimentaires > Microbiologie des denrées alimentaires > >]
Daube, Georges mailto [Université de Liège - ULg > Sciences des Denrées alimentaires > Microbiologie des denrées alimentaires >]
Gavini, Françoise [Institut Scientifique de Recherche Agronomique - INRA > Microbiologie et Chaîne Alimentaire, Villeneuve d'Ascq > > >]
2008
Journal of Applied Microbiology
Blackwell Publishing
104
1
276-284
Yes (verified by ORBi)
International
1364-5072
1365-2672
Oxford
United Kingdom
[en] Bifidobacterium choerinum ; Bifidobacterium pseudolongum ; Bifidobacterium thermophilum ; Chaperonin 60/genetics ; Colony Count, Microbial ; DNA-DNA hybridization ; Escherichia coli/isolation & purification ; faecal indicators ; hsp60 gene ; meat ; mupirocin ; PCR ; sheep ; slaughter
[en] Aims: The potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. The levels of bifidobacteria were compared with those of Escherichia coli. Total viable counts were followed along the chain (244 samples). Methods and Results: Forty-three per cent of the samples contained bifidobacteria, of which 15% were solely detected using a PCR method based on the hsp60 gene and not by a culture-based method. Bifidobacteria were detected in only three of nine sheep faeces samples using one or the other method. However, carcasses (types C and E) were highly contaminated. These sample types (30% and 28%, respectively) were positive for bifidobacteria and negative for E. coli. The species Bifidobacterium pseudolongum and Bif. thermophilum, isolated from faecal samples, were predominant. Bifidobacterium choerinum were found in C, D, E and F sample types. Conclusions: Bifidobacteria were shown more efficient than E. coli in carcasses samples. The presence of Bif. choerinum suggested a faecal pork contamination. Significance and Impact of the Study: Detection and identification of bifidobacteria, in correlation with E. coli counting, should improve hygiene quality of mutton processing chains.
Commission européenne : Direction générale de la Recherche
BIFID
Researchers
http://hdl.handle.net/2268/391
also: http://hdl.handle.net/2268/111748
10.1111/j.1365-2672.2007.03551.x

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