Reference : Validation of a two-plate microbiological method for screening antibiotic residues in sh...
Scientific journals : Article
Life sciences : Food science
http://hdl.handle.net/2268/38898
Validation of a two-plate microbiological method for screening antibiotic residues in shrimp tissue
English
Pham Kim, Dang mailto [Université de Liège - ULg > Sciences des denrées alimentaires > Anayse des denrées alimentaires > >]
Degand, Guy mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires > >]
Danyi, Sophie mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires > >]
Pierret, Gilles [ > > ]
Delahaut, Philippe [ > > ]
Vu Dinh, Ton [ > > ]
Maghuin-Rogister, Guy mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires > >]
Scippo, Marie-Louise mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > CART, Analyse des denrées alimentaires > >]
2010
Analytica Chimica Acta
Elsevier
672
30-39
Yes (verified by ORBi)
International
0003-2670
1873-4324
Amsterdam
The Netherlands
[en] Antibiotic residue ; Shrimp ; Microbiological method ; Sulfonamides ; (Fluoro)quinolones ; Tetracyclines
[en] Microbiological inhibition screening tests could play an important role to detect residues of antibiotics in the different animal food products, but very few are available for the aquaculture products in general, and for shrimps in particular. A two-plate microbiological method to screen shrimp for residues of the most commonly used antibiotics has been developed and validated according to criteria derived from the European Commission Decision 2002/657/CE. Bacillus subtilis was used as a sensitive strain to target antibiotics. Culture conditions on Petri plates (pH of medium) were selected to enhance the capacity of antibiotic detection. Antibiotic residues were extracted from shrimps using acetonitrile/acetone (70/30, v/v) before application on Petri plates seeded with B. subtilis. The method was validated using spiked blank tissues as well as antibiotic treated shrimps with enrofloxacin and tetracycline, two antibiotics often found to be used in shrimp production. For tetracyclines and (fluoro)quinolones, the detection capability was below the maximum residue limit (MRL), while it was around the MRL for sulfonamides. The specificity of the microbiological screening was 100% in all cases while the sensitivity and accuracy was 100% in almost all cases. The capacity of the method to detect contaminated samples was confirmed on antibiotic treated shrimps, analyzed in parallel with a confirmatory method (Liquid Chromatography coupled to mass spectrometry (LC–MS)).
Researchers
http://hdl.handle.net/2268/38898
10.1016/j.aca.2010.03.055
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TF4-4YRPDVF-3&_user=532038&_coverDate=04%2F01%2F2010&_alid=1345960409&_rdoc=3&_fmt=high&_orig=search&_cdi=5216&_sort=d&_docanchor=&view=c&_ct=29&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=815a98d48d9c29fdcc48ba06d3eaf9ec

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