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Identification of Psf, the Polypyrimidine Tract-Binding Protein-Associated Splicing Factor, as a Developmentally Regulated Neuronal Protein
Chanas-Sacre, Grazyna; Mazy-Servais, Cécile; Wattiez, Ruddy et al.
1999In Journal of Neuroscience Research, 57 (1), p. 62-73
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Keywords :
neuronal differentiation; neuronal splicing; radial glia; RC2 antibody
Abstract :
[en] The polypyrimidine tract-binding protein-associated splicing factor (PSF), which plays an essential role in mammalian spliceosomes, has been found to be expressed by differentiating neurons in developing mouse brain. The sequence of a fragment of mouse PSF was found to be remarkably similar to that of human PSF. Both the expression of PSF mRNA in cortex and cerebellum and PSF immunoreactivity in all brain areas were high during embryonic and early postnatal life and almost disappeared in adult tissue, except in the hippocampus and olfactory bulb where various neuronal populations remained PSF-immunopositive. Double-labeling experiments with anti-PSF antibody and anti-neurofilaments or anti-glial fibrillary acidic protein antibodies on sections of cortex, hippocampus, and cerebellum indicate that PSF is expressed by differentiating neurons but not by astrocytic cells. In vitro, mouse PSF was found to be expressed by differentiating cortical and cerebellar neurons. Radial glia or astrocyte nuclei were not immunopositive; however, oligodendrocytes differentiating in vitro were found to express PSF. The restricted expression of PSF suggests that this splicing factor could be involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation.
Disciplines :
Biochemistry, biophysics & molecular biology
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Chanas-Sacre, Grazyna;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Mazy-Servais, Cécile;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Wattiez, Ruddy;  Université de Mons-Hainaut - UMH > Department of Biological Chemistry
Pirard, Sandrine;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Rogister, Bernard  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Patton, James G;  Vanderbilt University > Department of Molecular biology
Belachew, Shibeshih ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie
Malgrange, Brigitte  ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Moonen, Gustave  ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine
Leprince, Pierre ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Language :
English
Title :
Identification of Psf, the Polypyrimidine Tract-Binding Protein-Associated Splicing Factor, as a Developmentally Regulated Neuronal Protein
Publication date :
01 July 1999
Journal title :
Journal of Neuroscience Research
ISSN :
0360-4012
eISSN :
1097-4547
Publisher :
John Wiley & Sons, Hoboken, United States - New York
Volume :
57
Issue :
1
Pages :
62-73
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FMRE - Fondation Médicale Reine Elisabeth [BE]
FWB - Fédération Wallonie-Bruxelles [BE]
Available on ORBi :
since 07 January 2009

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