Article (Scientific journals)
Stabilization and structure of telomeric and c-myc region intramolecular G-quadruplexes: The role of central cations and small planar ligands
Gabelica, Valérie; Baker, Erin Shammel; Teulade-Fichou, Marie-Paule et al.
2007In Journal of the American Chemical Society, 129 (4), p. 895-904
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Keywords :
mass spectrometry; ion mobility; G-quadruplex; ligand; DNA; structural biology
Abstract :
[en] A promising approach for anticancer strategies is the stabilization of telomeric DNA into a G-quadruplex structure. To explore the intrinsic stabilization of folded G-quadruplexes, we combined electrospray ionization mass spectrometry, ion mobility spectrometry, and molecular modeling studies to study different DNA sequences known to form quadruplexes. Two telomeric DNA sequences of different lengths and two DNA sequences derived from the NHE III1 region of the c-myc oncogene (Pu22 and Pu27) were studied. NH4+ and the ligands PIPER, TMPyP4, and the three quinacridines MMQ1, MMQ3, and BOQ1 were complexed with the DNA sequences to determine their effect on the stability of the G-quadruplexes. Our results demonstrate that G-quadruplex intramolecular folds are stabilized by NH4+ cations and the ligands listed. Furthermore, the ligands can be classified according to their ability to stabilize the quadruplexes and end stacking is shown to be the dominant mode for ligand attachment. In all cases our solvent-free experimental observations and theoretical modeling reveal structures that are highly relevant to the solution-phase structures.
Research center :
Giga-Systems Biology and Chemical Biology - ULiège
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Chemistry
Author, co-author :
Gabelica, Valérie ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie physique, spectrométrie de masse
Baker, Erin Shammel;  University of California at Santa Barbara - UCSB > Department of Chemistry and Biochemistry
Teulade-Fichou, Marie-Paule;  Collège de France, Paris > Laboratoire de Chimie des Interactions Moléculaires
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie physique, spectrométrie de masse
Bowers, Michael T;  University of California at Santa Barbara - UCSB > Department of Chemistry and Biochemistry
Language :
English
Title :
Stabilization and structure of telomeric and c-myc region intramolecular G-quadruplexes: The role of central cations and small planar ligands
Publication date :
2007
Journal title :
Journal of the American Chemical Society
ISSN :
0002-7863
eISSN :
1520-5126
Publisher :
Amer Chemical Soc, Washington, United States - Washington
Volume :
129
Issue :
4
Pages :
895-904
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 29 July 2008

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