Reference : Stabilization and structure of telomeric and c-myc region intramolecular G-quadruplex...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/384
Stabilization and structure of telomeric and c-myc region intramolecular G-quadruplexes: The role of central cations and small planar ligands
English
Gabelica, Valérie mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie physique, spectrométrie de masse >]
Baker, Erin Shammel [University of California at Santa Barbara - UCSB > Department of Chemistry and Biochemistry > > >]
Teulade-Fichou, Marie-Paule [Collège de France, Paris > > Laboratoire de Chimie des Interactions Moléculaires > >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie physique, spectrométrie de masse >]
Bowers, Michael T [University of California at Santa Barbara - UCSB > Department of Chemistry and Biochemistry > > >]
2007
Journal of the American Chemical Society
Amer Chemical Soc
129
4
895-904
Yes (verified by ORBi)
International
0002-7863
Washington
[en] mass spectrometry ; ion mobility ; G-quadruplex
[fr] ligand ; DNA ; structural biology
[en] A promising approach for anticancer strategies is the stabilization of telomeric DNA into a G-quadruplex structure. To explore the intrinsic stabilization of folded G-quadruplexes, we combined electrospray ionization mass spectrometry, ion mobility spectrometry, and molecular modeling studies to study different DNA sequences known to form quadruplexes. Two telomeric DNA sequences of different lengths and two DNA sequences derived from the NHE III1 region of the c-myc oncogene (Pu22 and Pu27) were studied. NH4+ and the ligands PIPER, TMPyP4, and the three quinacridines MMQ1, MMQ3, and BOQ1 were complexed with the DNA sequences to determine their effect on the stability of the G-quadruplexes. Our results demonstrate that G-quadruplex intramolecular folds are stabilized by NH4+ cations and the ligands listed. Furthermore, the ligands can be classified according to their ability to stabilize the quadruplexes and end stacking is shown to be the dominant mode for ligand attachment. In all cases our solvent-free experimental observations and theoretical modeling reveal structures that are highly relevant to the solution-phase structures.
Giga-Systems Biology and Chemical Biology ; Centre Interfacultaire d'Analyse des Résidus en Traces - CART
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers
http://hdl.handle.net/2268/384
10.1021/ja065989p

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