Reference : A twinned monoclinic crystal form of human peroxiredoxin 5 with eight molecules in the a...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/38172
A twinned monoclinic crystal form of human peroxiredoxin 5 with eight molecules in the asymmetric unit
English
Declercq, Jean-Paul mailto [Université Catholique de Louvain - UCL > Département de Chimie > Unité de Chimie Structurale (CSTR) > >]
Evrard, Christine mailto [Université Catholique de Louvain - UCL > Département de Chimie > Unité de Chimie Structurale (CSTR) > >]
2001
Acta Crystallographica Section D-Biological Crystallography
Blackwell Publishing
D57
1829-1835
Yes (verified by ORBi)
International
0907-4449
Oxford
United Kingdom
[en] The monoclinic crystal form of human peroxiredoxin 5 with eight molecules in the asymmetric unit was obtained under exactly the same conditions as the tetragonal form with one molecule in the asymmetric unit, except that the latter was briefly cryosoaked with halide for derivatization. A merohedral twinning was observed, which is rather unusual in the monoclinic system and only possible with particular unit-cell dimensions. After detwinning the native and a mercury derivative, the structure was solved by the SIR method with the help of the non-crystallographic symmetry. The packing of the monoclinic and tetragonal forms are compared, with special attention to the role of bromide ions in the change of space group after crystallization. The availability of nine (eight monoclinic plus one tetragonal) independent molecules allows an analysis of the mobility. The two Cys residues implicated in the peroxide-reduction mechanism are located in rigid regions but are covered by mobile loops.
http://hdl.handle.net/2268/38172

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