Article (Scientific journals)
Kinetics and Thermodynamics of Glucose Isomerase Crystallization
Sleutel, Mike; Willaert, Ronnie; Gillespie, Christopher et al.
2009In Crystal Growth and Design, 9, p. 497-504
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Abstract :
[en] A quantitative study using laser confocal microscopy combined with differential interference microscopy on the kinetics and thermodynamics of the crystallization of glucose isomerase is presented. Fundamental crystallization parameters are determined from the kinetics of step advancement and rates of two-dimensional (2D) nucleation. The ruling mass transfer pathway and accompanying activation barriers are discussed. In brief, the solubility exhibits normal temperature dependence and the crystallization enthalpy is the thermodynamic driving force. The diminishing entropic cost for higher PEG concentrations is attributed to water structuring and a decrease in water activity. The prominent step generation mechanism is homogeneous 2D nucleation for high supersaturations. At low driving forces 2D nucleation occurs on anomalously hyperactive sites and the step edge free energies for homogeneous and heterogeneous nucleation are determined. The number of nucleation centers for both mechanisms are estimated and from the density of nucleation centers we obtain for the activation barrier of adsorption ∼3.8 kJ mol-1. No step-step interaction is observed for interstep distances >70 nm. Theoretical fits of step velocity data suggest surface diffusion makes a non-negligible contribution to surface kinetics. From the temperature dependence of the step kinetic coefficient the activation barrier for crystallization was determined to be <22.4 kJ mol-1.
Disciplines :
Physics
Author, co-author :
Sleutel, Mike;  Vrije Universiteit Brussel - VUB
Willaert, Ronnie;  Vrije Universiteit Brussel - VUB
Gillespie, Christopher;  University of Delaware
Evrard, Christine ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Wyns, Lode;  Vrije Universiteit Brussel - VUB
Maes, Dominique;  Vrije Universiteit Brussel - VUB
Language :
English
Title :
Kinetics and Thermodynamics of Glucose Isomerase Crystallization
Publication date :
2009
Journal title :
Crystal Growth and Design
ISSN :
1528-7483
eISSN :
1528-7505
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Volume :
9
Pages :
497-504
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 May 2010

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