Reference : Unexpected Influence of a C-Terminal-Fused His-Tag on the Processing of an Enzyme and on...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/3800
Unexpected Influence of a C-Terminal-Fused His-Tag on the Processing of an Enzyme and on the Kinetic and Folding Parameters
English
Ledent, Philippe [>ULg > > > > Centre d'Ingénierie des Protéines (CIP) > >]
Duez, Colette mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Fonze, Eveline [>ULg > > > > CIP > >]
Charlier, Paulette mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Rhazi-Filali, Fouzia [>Université de Meknes, Morocco > > >Faculté des Sciences > > >]
Thamm, Iris mailto [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
Samyn, Bart [>RUG > > >Vakgroep Biochemie, Fysiologie en Microbiologie > > >]
Devreese, Bart [>RUG > > >Vakgroep Biochemie, Fysiologie en Microbiologie > > >]
Van Beeumen, Jozef [>RUG > > >Vakgroep Biochemie, Fysiologie en Microbiologie > > >]
Lamotte-Brasseur, Josette [>ULg > > > > CIP > >]
Frère, Jean-Marie [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
Vanhove, Marc [ULg > > CIP > >]
Guillaume, Gilliane [ULg > > CIP > >]
Lejeune, Annabelle [ULg > > CIP > >]
18-Aug-1997
FEBS Letters
413
2
194-6
International
0014-5793
[en] The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this type of protein engineering might not always be as 'neutral' as generally assumed.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/3800

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