Article (Scientific journals)
Angiopoietin/Tie2 signaling transforms capillaries into venules primed for leukocyte trafficking in airway inflammation.
Fuxe, Jonas; Lashnits, Erin; O'Brien, Shaun et al.
2010In American Journal of Pathology, 176 (4), p. 2009-18
Peer Reviewed verified by ORBi
 

Files


Full Text
Submitted PDF AJP09-2150T_1.pdf
Publisher postprint (887.89 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Vascular endothelial growth factor (VEGF) is a key angiogenic factor in tumors, but less is known about what drives vascular remodeling in inflammation, where plasma leakage and leukocyte influx are prominent features. In chronic airway inflammation in mice infected by the bacterium Mycoplasma pulmonis (M. pulmonis), the segment of the microvasculature that supports leukocyte adhesion and migration expands through remodeling of capillaries into vessels with features of venules. Here, we report that the angiopoietin/Tie2 pathway is an essential driving force for capillary remodeling into venules in M. pulmonis-infected mouse airways. Similar to M. pulmonis infection, systemic overexpression of angiopoietin-1 (Ang1) resulted in remodeling of airway capillaries into venular-like vessels that expressed venous markers like P-selectin, ICAM-1, and EphB4 and were sites of leukocyte adhesion during lipopolysaccharide-induced acute inflammation. Ang1 and Ang2 protein increased in M. pulmonis-infected mouse airways but came from different cellular sources: Ang1 was expressed in infiltrating neutrophils and Ang2 in endothelial cells. Indeed, systemic administration of soluble Tie2 inhibited capillary remodeling, induction of venous markers, and leukocyte influx in M. pulmonis-infected mouse airways. Together, these findings suggest that blockade of the Ang/Tie2 pathway may represent a therapeutic approach in airway inflammation.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Fuxe, Jonas
Lashnits, Erin
O'Brien, Shaun
Baluk, Peter
Tabruyn, Sébastien ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Kuhnert, Frank
Kuo, Calvin
Thurston, Gavin
McDonald, Donald M
Language :
English
Title :
Angiopoietin/Tie2 signaling transforms capillaries into venules primed for leukocyte trafficking in airway inflammation.
Publication date :
2010
Journal title :
American Journal of Pathology
ISSN :
0002-9440
eISSN :
1525-2191
Publisher :
American Society for Investigative Pathology, Bethesda, United States - Maryland
Volume :
176
Issue :
4
Pages :
2009-18
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 May 2010

Statistics


Number of views
53 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
29
Scopus citations®
without self-citations
21
OpenCitations
 
25

Bibliography


Similar publications



Contact ORBi